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The effect of polyamines and silver thiosulphate on micropropagation of date palm followed by genetic stability assessment

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Abstract

There are some limitations in date palm micropropagation. These include low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the study was to determine the effect of the polyamines and Silver Thiosulphate (STS) on the enhancement of shoot multiplication and genetic stability of in vitro cultures of date palm cultivar Quntar. Media supplemented with 75 mg L−1 SPD in combination with 10 mg L−1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective on root formation and the number of roots per shoot, where the best result, 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mg L−1 PUT and 10 mg L−1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 80% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar was beneficial to improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

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Abbreviations

NAA:

Naphthaleneacetic acid;

IAA:

Indoleacetic acid

2iP:

N6-(2-Isopentenyl) adenine

PUT:

Putrescine

SPD:

Spermidine

STS:

Silver thiosulfate

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Acknowledgements

The author wishes to thank all the Date Palm Research Center staff, especially in the Date Palm propagation Lab.

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  1. Date Palm Research Centre, University of Basrah, Basrah, Iraq

    Ahmed Madi Waheed Al-Mayahi

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  1. Ahmed Madi Waheed Al-Mayahi
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AMWA preparing the culture media and the conduct of plant tissue culture of the date palm, and the follow of the growth and development of cultures. The author also analyzed the physiological characteristics of the tissues and wrote the manuscript.

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Correspondence to Ahmed Madi Waheed Al-Mayahi.

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Al-Mayahi, A.M.W. The effect of polyamines and silver thiosulphate on micropropagation of date palm followed by genetic stability assessment. World J Microbiol Biotechnol 38, 124 (2022). https://doi.org/10.1007/s11274-022-03305-5

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