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In vitro toxicological evaluation of domestic effluent treated by formulated synthetic autochthonous bacterial consortium

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Abstract

Supplementation of consortium comprising of aboriginal bacterial species with high degradation capacity can significantly enhance the biodegradation process of the domestic wastewater. The present study examined the bioremediation of domestic wastewater using a novel bacterial consortium comprising of five autochthonous bacterial strains with high potential for reduction in BOD, COD and protein content to 89%, 55% and 86%, respectively after 24 h of incubation. HPLC and GC–MS analyses revealed that the chosen consortium had successfully degraded wide-ranging complex organic compounds, which is crucial in the decontamination of wastewater. Phytotoxicity assay of the effluent exhibited that the seeds of Vigna radiata showed better growth and germination when subjected to wastewater treated by novel bacterial consortium as compared to the seeds exposed to untreated wastewater. Further, raw and treated wastewater were assessed for their genotoxicity with comet assay which displayed the intensity of DNA damage in the Allium cepa root tip cells before and after exposure to treated effluent. It is evident from the demonstrated results that the formulated bacterial consortium can be used successfully in a small-scale wastewater treatment plant.

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Acknowledgements

The authors are grateful to Amity Institute of Biotechnology, Amity University Uttar Pradesh, for providing the laboratory facilities and funds to carry out the work smoothly. The authors are thankful to Mr. Surya Pratap Singh Shakya, Amity Institute of Pharmacy, Amity University Uttar Pradesh for doing HPLC analysis, Mr. Ajay Kumar, AIRF, JNU, New Delhi for doing GC–MS analysis and Dr. Saras Jyoti, Research Associate, Amity Institute of Molecular Medicine and Stem Cell Research, Amity University Uttar Pradesh for doing the confocal fluorescence microscopy. The authors are also grateful to the reviewers for their suggestions that helped immensely in giving the manuscript its present form.

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Authors and Affiliations

  1. Water Quality Monitoring and Bioremediation Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, 201313, India

    Tithi Mehrotra & Rachana Singh

  2. Central Road Research Institute (CSIR-CRRI), New Delhi, 110025, India

    Anuradha Shukla

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  1. Tithi Mehrotra
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  2. Anuradha Shukla
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  3. Rachana Singh
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Correspondence to Rachana Singh.

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Mehrotra, T., Shukla, A. & Singh, R. In vitro toxicological evaluation of domestic effluent treated by formulated synthetic autochthonous bacterial consortium. World J Microbiol Biotechnol 35, 184 (2019). https://doi.org/10.1007/s11274-019-2756-0

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