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Accelerated removal of Sudan dye by Shewanella oneidensis MR-1 in the presence of quinones and humic acids

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Abstract

Although there have been many studies on bacterial removal of soluble azo dyes, much less information is available for biological treatment of water-insoluble azo dyes. The few bacterial species capable of removing Sudan dye generally require a long time to remove low concentrations of insoluble dye particles. The present work examined the efficient removal of Sudan I by Shewanella oneidensis MR-1 in the presence of redox mediator. It was found that the microbially reduced anthraquinone-2,6-disulfonate (AQDS) could abiotically reduce Sudan I, indicating the feasibility of microbially-mediated reduction. The addition of 100 μM AQDS and other different quinone compounds led to 4.3–54.7 % increase in removal efficiencies in 22 h. However, adding 5-hydroxy-1,4-naphthoquinone into the system inhibited Sudan I removal. The presence of 10, 50 and 100 μM AQDS stimulated the removal efficiency in 10 h from 26.4 to 42.8, 54.9 and 64.0 %, respectively. The presence of 300 μM AQDS resulted in an eightfold increase in initial removal rate from 0.19 to 1.52 mg h−1 g−1 cell biomass. A linear relationship was observed between the initial removal rates and AQDS concentrations (0–100 μM). Comparison of Michaelis–Menten kinetic constants revealed the advantage of AQDS-mediated removal over direct reduction. Different species of humic acid could also stimulate the removal of Sudan I. Scanning electronic microscopy analysis confirmed the accelerated removal performance in the presence of AQDS. These results provide a potential method for the efficient removal of insoluble Sudan dye.

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Acknowledgments

The work was financially supported by National Natural Science Foundation of China (No. 51008044), Fundamental Research Funds for the Central Universities, and China Postdoctoral Science Foundation (No. 201104596).

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Authors and Affiliations

  1. Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China

    Guangfei Liu, Jiti Zhou, Qiuyan Ji, Jing Wang, Ruofei Jin & Hong Lv

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  1. Guangfei Liu
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  2. Jiti Zhou
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  3. Qiuyan Ji
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  4. Jing Wang
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  5. Ruofei Jin
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Correspondence to Jing Wang.

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Liu, G., Zhou, J., Ji, Q. et al. Accelerated removal of Sudan dye by Shewanella oneidensis MR-1 in the presence of quinones and humic acids. World J Microbiol Biotechnol 29, 1723–1730 (2013). https://doi.org/10.1007/s11274-013-1336-y

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