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Anaerobic biodecolorization mechanism of methyl orange by Shewanella oneidensis MR-1

  • Environmental biotechnology
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Abstract

In this work, we investigated the anaerobic decolorization of methyl orange (MO), a typical azo dye, by Shewanella oneidensis MR-1, which can use various organic and inorganic substances as its electron acceptor in natural and engineered environments. S. oneidensis MR-1 was found to be able to obtain energy for growth through anaerobic respiration accompanied with dissimilatory azo-reduction of MO. Chemical analysis shows that MO reduction occurred via the cleavage of azo bond. Block of Mtr respiratory pathway, a transmembrane electron transport chain, resulted in a reduction of decolorization rate by 80%, compared to the wild type. Knockout of cymA resulted in a substantial loss of its azo-reduction ability, indicating that CymA is a key c-type cytochrome in the electron transfer chain to MO. Thus, the MtrA-MtrB-MtrC respiratory pathway is proposed to be mainly responsible for the anaerobic decolorization of azo dyes such as MO by S. oneidensis.

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Acknowledgements

This work was partially supported by the National Natural Science Foundation of China (20907050) and the Outstanding Young Scientists Foundation of Anhui Province, China (10040606Y27). The authors wish to thank Professor K. H. Nealson from University of Southern California for providing the bacteria used in this work.

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Authors and Affiliations

  1. Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China

    Pei-Jie Cai, Xiang Xiao, Yan-Rong He, Wen-Wei Li, Jian Chu, Chao Wu, Meng-Xing He, Zhe Zhang, Guo-Ping Sheng, Fang Xu & Han-Qing Yu

  2. Advanced Laboratory for Environmental Research & Technology, USTC-CityU, Suzhou, 215123, China

    Pei-Jie Cai, Wen-Wei Li, Michael Hon-Wah Lam & Han-Qing Yu

  3. School of Environment, Jiangsu University, Zhenjiang, 212013, China

    Xiang Xiao

  4. Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong

    Pei-Jie Cai & Michael Hon-Wah Lam

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  1. Pei-Jie Cai
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  2. Xiang Xiao
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Correspondence to Xiang Xiao or Han-Qing Yu.

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Cai, PJ., Xiao, X., He, YR. et al. Anaerobic biodecolorization mechanism of methyl orange by Shewanella oneidensis MR-1. Appl Microbiol Biotechnol 93, 1769–1776 (2012). https://doi.org/10.1007/s00253-011-3508-8

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  • DOI: https://doi.org/10.1007/s00253-011-3508-8

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