Abstract
Liquid residues from beer (RB) and potato (RP) processing were evaluated as carbon sources for the production of docosahexaenoic acid (C22:6n-3, DHA) by two native Thraustochytriidae sp., M12-X1 and C41, in shaking flask experiments. Results were compared with those obtained in the fermentations of glucose, maltose, soluble starch and ethanol. Both strains produced the highest biomass concentration (2.3 g/L) in the fermentation of RB supplemented with nitrogen sources [yeast extract (YE) and monosodium glutamate (MSG)]. DHA content in the fatty acids produced by the native thraustochytrids was dependent on the fermented carbon source; the fatty acids from biomass grown on carbon sources that permitted a lower growth rate contained more DHA. The highest DHA productivity [55.1 mg/(day L)] was obtained in the fermentation of RB-YE-MSG by M12-X1 strain. In this medium, M12-X1 strain grew at a specific growth rate of 0.014 h−1 and total fatty acid content in the biomass was 41.3%. Production of DHA by M12-X1 strain followed a non-growth rate associated pattern and DHA content in the biomass decreased significantly after growth ceased.
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Acknowledgments
This work is part of the doctoral thesis of MSc Benita Quilodran at Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, Chile. The authors acknowledge the economical support provided by Dirección de Investigación, Universidad de Los Lagos through Project No 0001906 and Project MECESUP ULA 0202. The authors also acknowledge Mr. Alberto Peters (Casa Cervecera Puerto Montt) for providing the brewery by-product.
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Quilodrán, B., Hinzpeter, I., Quiroz, A. et al. Evaluation of liquid residues from beer and potato processing for the production of docosahexaenoic acid (C22:6n-3, DHA) by native thraustochytrid strains. World J Microbiol Biotechnol 25, 2121–2128 (2009). https://doi.org/10.1007/s11274-009-0115-2
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DOI: https://doi.org/10.1007/s11274-009-0115-2