Skip to main content
SpringerLink
Log in

Optimization of production of docosahexaenoic acid (DHA) byThraustochytrium aureum ATCC 34304

  • Published:
Journal of the American Oil Chemists’ Society

Abstract

By varying culture carbon source, lipid content in mycelium ofThraustochytrium aureum ATCC 34304 varied widely in the range 1–25% of biomass weight. Docosahexaenoic acid (DHA) content of mycelium lipid was higher (65–76%) when biomass lipid content was very low (1–2%) and lower (40–50%) when biomass contained a high lipid content (14–18%). DHA yields from glucose, starch and maltose were 270, 325 and 334 mg/L, respectively. DHA yield and content of biomass was optimal at an initial culture pH of 6.0. During the culture cycle ofT. aureum, DHA content in lipids remained relatively constant with optimal DHA yield being observed after six days. Biomass, lipid content in biomass, DHA content in biomass and DHA yield were all optimal at a cultivation temperature of 28°C. However, the proportion of DHA in lipids declined with increase in temperature. Biomass, lipids in biomass and DHA yields were 13%, 42% and 47% higher, respectively, din light-exposed cultures as compared to dark cultures. A maximum yield of DHA of 511 mg/L was observed in light- exposed cultures containing 2.5% starch, where lipids accounted for over 20% of biomass dry weight.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dyerberg, J., H.O. Bang, E. Stoffersen, S. Moncada and J.R. Vane,Lancet ii:117 (1978).

  2. Dyerberg, J.,Nutr. Rev. 44:125 (1986).

    Article  CAS  Google Scholar 

  3. Braden, L.M., and K.K. Carroll,Lipids 21:285 (1986).

    Article  CAS  Google Scholar 

  4. Kremer, J.M.,J. Bigauaoette, A.V. Michalek, M.A. Timchalk, L. Lininger, R.I. Rynes, C. Hucyck, J. Zieminski and L.E. Bar- tholomew,Lancet i:184 (1985).

    Article  Google Scholar 

  5. Dratz, E.A.,and A.J. Deese, in:Health Effects of Polyunsaturated Fatty Acid in Seafoods, edited by A.P. Simopoulos, R.R. Kifer and R.E. Martin, Academic Press Ina, Orlando, 1986, pp. 319–330.

    Google Scholar 

  6. Neuringer, M., W.E. Connor, C. van Patten and L. J. Barstad,Clin Invest 73:272 (1984).

    CAS  Google Scholar 

  7. Salem, N., Jr., H-Y. Kim and J. Yargey, in:Health Effects of Polyunsaturated Fatty Acids in Seafoods, edited by A.P. Simopoulos, R.R. Kifer and R.E. Martin, Academic Press, Orlan- do, 1986, pp. 49–60.

    Google Scholar 

  8. Simopoulos, A.P., in:Health and Disease, edited by R.S. Lees and M. Karel, Dekker, New York, 1990.

    Google Scholar 

  9. Anderson, R.E.,Exp. Eye Res. 10:339 (1970).

    Article  CAS  Google Scholar 

  10. O’Brian, J.S., and E.L. Sampson,J. Lipid Res. 6:545 (1965).

    Google Scholar 

  11. Poulos, A., A. Darin-Bennett and I.C. White,Comp. Biochem. Physiol. 46B:541 (1975).

    Google Scholar 

  12. Haagsma, N., C.M. van Gent, J.B. Luten, R.W. de Jong and E. van Doom,J. Am. Oil Chem. Soc. 59:117 (1982).

    Article  CAS  Google Scholar 

  13. Contreras, R.O., O.A. Migiaro and R.A. Raffo,Ibid 48:98 (1971).

    Article  Google Scholar 

  14. Fujita, T., and M. Makuta, U.S. Patent 4,377,526 (1983).

  15. Aveldan, M.I., M. van Rollins and L.A. Harrocks,J. Lipid Res. 24:83 (1983).

    Google Scholar 

  16. Nilsson, W.B., J. Ganglitz, J.K. Hudson, V.F. Stout and J. Spinelli,J. Am. Oil Chem. Soc. 65:109 (1988).

    Article  CAS  Google Scholar 

  17. Dunham, J.E., G.W. Harrington and G.G. Holz, Jr.,Biol. Bull. 131:389 (1966).

    Google Scholar 

  18. Yongmanitchai, W., and O.P. Ward,Proc. Biochem. 24:117 (1989).

    CAS  Google Scholar 

  19. Sajbidor, J., S Dobronova and M. Certik,Biotechnol. Lett. 12:455 (1990).

    Article  CAS  Google Scholar 

  20. Shinmen, Y., S. Shimizu, K. Akimoto and H. Yamada,Appl. Microbiol. Biotechnol. 31:11 (1989).

    Article  CAS  Google Scholar 

  21. Totani, N., and K. Oba,Lipids 22:1060 (1987).

    Article  CAS  Google Scholar 

  22. Sinden, K.W.,Eng. Microbial Tech. 9:124 (1987).

    Article  Google Scholar 

  23. Haskins, R.H., A.P. Lulloch and R.G. Micetich,Can. J. Microbiol 10:187 (1964).

    Article  Google Scholar 

  24. Goldstein, S.,Mycol. 55:799 (1963).

    Article  CAS  Google Scholar 

  25. Goldstein, S.,Am. J. Bot. 50:271 (1963).

    Article  CAS  Google Scholar 

  26. Bligh. E.G., and W.J. Dyer,Can. J. Biochem. Physiol 37:911 (1959).

    CAS  Google Scholar 

  27. Holub, B.J., and C.M. Skeaff,Meth. Enzymol. 141:234 (1987).

    Article  CAS  Google Scholar 

  28. Prill, E.A., P.R. Wensk and W.H. Peterson, III,Biochem. J. 29:21 (1935).

    CAS  Google Scholar 

  29. Kessell, R.H.J.,J. Appl. Bacteriol. 31:220 (1968).

    CAS  Google Scholar 

  30. Cantreall, H.F., and W.M. Dowler,Mycol. 63:31 (1971).

    Article  Google Scholar 

  31. Wassef, M.K., in:Advances in Lipid Research, edited by A. Paolet- ti and D. Kritchevsky, Academic Press, New York, 1977, pp. 159–232.

    Google Scholar 

  32. Singh, J., and T.K. Walker,Biochem. J. 62:286 (1956).

    CAS  Google Scholar 

  33. Boulton, CA., and C. Ratledge, in:Comprehensive Biotechnology, Vol. 1: The Principles of Biotechnology, Scientific Fundamentals, edited by M. Moo-Young, Pergamon, Oxford, 1985, p. 459.

    Google Scholar 

  34. Gregory, M., and M. Woodbine,J. Exper. Bot. 4:314 (1953).

    Article  CAS  Google Scholar 

  35. Kates, M., and R.M. Baxter,Can. J. Biochem. Physiol. 40:1213 (1962).

    CAS  Google Scholar 

  36. Shaw, R.,Adv. Lipid Res. 4:7 (1966).

    Google Scholar 

  37. Gad, A.M., and M.M. Hassan,J. Chem. United Arab Republic 7:31 (1964).

    CAS  Google Scholar 

  38. Bowman, R.D., and R.O. Mumma,Biochim. Biophys. Acta 144:501 (1967).

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. P. K. Bajpai & P. Bajpai

    Present address: Thapar Corporate Research & Development Centre, Patiala, India

Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    P. K. Bajpai, P. Bajpai & O. P. Ward

Authors
  1. P. K. Bajpai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Bajpai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. P. Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

To whom correspondence should be addressed.

About this article

Cite this article

Bajpai, P.K., Bajpai, P. & Ward, O.P. Optimization of production of docosahexaenoic acid (DHA) byThraustochytrium aureum ATCC 34304. J Am Oil Chem Soc 68, 509–514 (1991). https://doi.org/10.1007/BF02663823

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02663823

Key Words

Search

Navigation