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Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08

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Abstract

The effect of the addition of maltitol on the production of raw starch digesting glucoamylase (RSDG) by Aspergillus niger F-08 was studied in the paper. The activity of RSDG formed by Aspergillus niger F-08 was enhanced dramatically by the addition of maltitol to the medium and it was confirmed that maltitol acted as a very efficient inducer for RSDG production. As a result, with the addition of 8 g maltitol in 1 l basal medium at the beginning of the culture, the enzyme activity was enhanced near 8-fold. The synthesis mechanism of RSDG by Aspergillus niger F-08 was also studied on molecular level. It was found that the regulation of RSDG synthesis by Aspergillus niger F-08 occurred at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of maltitol at transcriptional level.

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Authors and Affiliations

  1. State Key Laboratory of Tropical Corp Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Haiyan Sun, Pingjuan Zhao & Ming Peng

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  1. Haiyan Sun
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  2. Pingjuan Zhao
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  3. Ming Peng
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Correspondence to Ming Peng.

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Sun, H., Zhao, P. & Peng, M. Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08. World J Microbiol Biotechnol 24, 2613–2618 (2008). https://doi.org/10.1007/s11274-008-9785-4

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  • DOI: https://doi.org/10.1007/s11274-008-9785-4

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