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Genetic transformation of the sugar beet plastome

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Abstract

It is very important for the application of chloroplast engineering to extend the range of species in which this technology can be achieved. Here, we describe the development of a chloroplast transformation system for the sugar beet (Beta vulgaris L. ssp. vulgaris, Sugar Beet Group) by biolistic bombardment of leaf petioles. Homoplasmic plastid-transformed plants of breeding line Z025 were obtained. Transformation was achieved using a vector that targets genes to the rrn16/rps12 intergenic region of the sugar beet plastome, employing the aadA gene as a selectable marker against spectinomycin and the gfp gene for visual screening of plastid transformants. gfp gene transcription and protein expression were shown in transplastomic plants. Detection of GFP in Comassie blue-stained gels suggested high GFP levels. Microscopy revealed GFP fluorescence within the chloroplasts. Our results demonstrate the feasibility of engineering the sugar beet chloroplast genome; this technology provides new opportunities for the genetic improvement of this crop and for social acceptance of genetically modified sugar beet plants.

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Acknowledgements

This article is contribution no. 130 from the Institute of Plant Genetic, Research Division of Perugia. This work was supported by the European Framework VI Specific Target Research Project “Transcontainer”. We are grateful to Dr. Gunter Diener (KWS SAAT AG) for providing us the monogerm sugar beet seeds and Dr. Marco De Biaggi and Dr. Enrico Biancardi (CRA – CIN Centro di Ricerca per le Colture Industriali Sede di Rovigo) for the other sugar beet breeding lines. We thank Professor Henry Daniell for providing pCR 2.1-5′UTR and pLD-CtV, and Dr. Phil Dix and Dr. Matthew McCabe for their helpful comments.

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Authors and Affiliations

  1. Institute of Plant Genetics – Research Division of Perugia, Italian National Research Council (CNR), via della Madonna Alta, 130, 06128, Perugia, Italy

    Francesca De Marchis, Yongxin Wang, Sergio Arcioni & Michele Bellucci

  2. Institute of Animal Science, CAAS, Yuan Ming Yuan, West Road, Haidian, Beijing, 100094, China

    Yongxin Wang

  3. Dip. to Biotechnologie Agrarie, Università degli Studi di Padova, v.le dell’Università, 16, Legnaro (PD), 35020, Italy

    Piergiorgio Stevanato

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  1. Francesca De Marchis
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  2. Yongxin Wang
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  4. Sergio Arcioni
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  5. Michele Bellucci
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Correspondence to Michele Bellucci.

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De Marchis, F., Wang, Y., Stevanato, P. et al. Genetic transformation of the sugar beet plastome. Transgenic Res 18, 17–30 (2009). https://doi.org/10.1007/s11248-008-9193-4

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