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Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L.

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Abstract

Prunella vulgaris L. is a very important species with worldwide traditional medicinal uses. Despite of its medicinal importance, there is lack of research efforts on cultivation and phytochemicals production of this plant. In the present study, an effort has been made to optimize various culture conditions for establishment of cell suspension culture and production of desired secondary metabolites. Initially, callus was induced from petiole explants on Murashige and Skoog medium supplemented with 2.0 mg/l naphthalene acetic acid (NAA) in dark. For establishment of suspension culture, friable calli were shifted to liquid medium fortified with 2.0 mg/l NAA. The differential effects of sucrose (5–50 g/l), pH (5–6) and different photoperiod regimes on suspension biomass, secondary metabolites accumulation and antioxidant activities were investigated. Maximum levels of biomass, phenolics, flavonoids, protein content and antioxidant activities were displayed by cultures treated with 20–25 g/l of sucrose. Similarly, a lower pH of 5.3 resulted in enhanced biomass, phenolics, flavonoids, protein content and antioxidant activities. Furthermore, the cultures grown under 12D/18L, 14D/16L and 16D/14L photoperiods exhibited higher levels of biomass and secondary metabolites, compared to control (16L/8D). These investigations conclude that sucrose, different pH levels and photoperiod regimes modulate cell growth and the production of bioactive compounds in cell suspension cultures of P. vulgaris.

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References

  • Ali M, Abbasi BH (2014) Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. J Photochem Photobiol B: Biol 140:223–227

    Article  CAS  Google Scholar 

  • Ali MB, Khatun S, Hahn EJ, Paek KY (2006) Enhancement of phenylpropanoid enzymes and lignin in Phalaenopsis orchid and their influence on plant acclimatization at different levels of photosynthetic photon flux. Plant Growth Regul 49:137–146

    Article  CAS  Google Scholar 

  • Baque MA, Elgirban A, Lee EJ, Paek KY (2012) Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.). Acta Physiol Plant 34:405–415

    Article  CAS  Google Scholar 

  • Barz W, Daniel S, Hinderer W, Jaques U, Kessmann H, Koster J, Tiemann K (1988) Elicitation and metabolism of phytoalexins in plant cell cultures. In: Pais M, Mavituna F, Novais J (eds) Plant cell biotechnol. NATO ASI Series. Springer, Berlin, pp 211–230

    Chapter  Google Scholar 

  • Bhakuni RS, Jain DC, Sharma RP, Kumar S (2001) Secondary metabolites of Artemisia annua and their biological activity. Curr Sci 80:35–48

    CAS  Google Scholar 

  • Bidchol AM, Wilfred A, Abhijna P, Harish R (2011) Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. Food Bioproc Tech 4:1137–1143

    Article  Google Scholar 

  • Bourgaud F, Gravot A, Milesi S, Gonteir E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851

    Article  CAS  Google Scholar 

  • Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT (2005) Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J Sci Food Agric 85:265–272

    Article  CAS  Google Scholar 

  • Chen Y, Yu M, Zhu Z, Zhang L, Guo Q (2013) Optimisation of potassium chloride nutrition for proper growth, physiological development and bioactive component production in Prunella vulgaris L. PLoS ONE 8:1–7

    CAS  Google Scholar 

  • Cheung HY, Zhang QF (2008) Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers. J Chromatogr A 1213:231–238

    Article  CAS  PubMed  Google Scholar 

  • Cui XH, Murthy HM, Wu CH, Paek KY (2010) Sucrose induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell, Tissue Organ Cult 103:7–14

    Article  CAS  Google Scholar 

  • Eilert U (1987) Elicitation: methodology and aspects of application. In: Constabel F, Vasil I (eds) Cell culture and somatic cell genetics of plants, vol 4. Academic Press, San Diego, pp 153–196

    Google Scholar 

  • Fazal H, Abbasi BH, Ahmad N (2014) Optimization of adventitious root culture for production of biomass and secondary metabolites in Prunella vulgaris L. Appl Biotechnol Biochem 174:2086–2095

    Article  CAS  Google Scholar 

  • Feng L, Au-yeung W, Xu YH, Wang SS, Quan Zhu Q, Xiang P (2011) Oleanolic acid from Prunella vulgaris L. induces SPC-A-1 cell line apoptosis via regulation of bax, bad and Bcl-2 expression. Asian Pac J Cancer Prev 12:403–408

    PubMed  Google Scholar 

  • Ferri M, Righetti L, Tassoni A (2011) Increasing sucrose concentrations promote phenylpropanoid biosynthesis in grapevine cell cultures. J Plant Physiol 168:189–195

    Article  CAS  PubMed  Google Scholar 

  • Ghasemzadeh A, Jaafar HZE, Rahmat A, Wahab PEM, Halim MRA (2010) Effect of different light intensities on total phenolics and flavonoids synthesis and antioxidant activities in young ginger varieties (Zingiber officinale Roscoe). Int J Mol Sci 11:3885–3897

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Giri L, Dhyani P, Rawat S, Bhatt ID, Nandi SK, Rawal RS, Pande V (2012) In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: a rare Himalayan medicinal orchid. Ind Crops Prod 39:1–6

    Article  CAS  Google Scholar 

  • Golembiovska OI, Tsurkan AA (2013) Anthocyanins profiling of Prunella vulgaris L. grown in Ukraine. Pharm Innov J 2:42–48

    CAS  Google Scholar 

  • Hohe A, Reski R (2002) Optimization of a bioreactor culture of the moss Physcomitrella patens for mass production of proptoplasts. Plant Sci 0:1–6

    Google Scholar 

  • Huang R, Zhao M, Yang X, Huang J, Yang Y, Chen B, Tan J, Huang J, Li Z, Lv Y, Ji G (2013) Effects of Prunella vulgaris on the mice immune function. PLoS ONE 8:1–8

    Article  Google Scholar 

  • Khan MA, Abbasi BH, Ahmed N, Ali H (2013) Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Ind Crops Prod 46:105–110

    Article  CAS  Google Scholar 

  • Kim HJ, Chen F, Xi W, Choi JH (2006) Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.). J Agric Food Chem 54:7263–7269

    Article  CAS  PubMed  Google Scholar 

  • Lagrimini LM (1991) Wound-induced deposition of polyphenols in transgenic plants over expressing peroxidase. Plant Physiol 96:577–583

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Liu GM, Jia XB, Wang HB, Feng L, Chen Y (2009) Review about current status of cancer prevention for the chemical composition or composition and function mechanism of Prunella vulgaris. J Chin Med Mater 3:1920–1926

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Meratan AA, Gaffari SM, Nikram V (2009) In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum. Biol Plant 53:5–10

    Article  CAS  Google Scholar 

  • Morkunas I, Marczak L, Stachowiak J, Stobiecki M (2005) Sucrose-induced lupine defense against Fusarium oxysporum sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum. Plant Physiol Biochem 43:363–373

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nayyar H, Gupta D (2006) Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environ Exp Bot 58:106–113

    Article  CAS  Google Scholar 

  • Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153

    Article  CAS  PubMed  Google Scholar 

  • Rasool R, Ganai BA, Akbar S, Kamili AN, Masood A (2010) Phytochemical screening of Prunella vulgaris L. an important medicinal plant of Kashmir. Pak J Pharm Sci 23(4):399–402

    CAS  PubMed  Google Scholar 

  • Riedel H, Cai Z, Kütük O, Smetanska I (2010) Obtaining phenolic acids from cell cultures of various Artemisia species. Afr J Biotechnol 9:8805–8809

    CAS  Google Scholar 

  • Sakamoto A, Murata N (2002) The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant, Cell Environ 25:163–171

    Article  CAS  Google Scholar 

  • See KS, Bhatt A, Keng CL (2011) Effect of sucrose and methyl jasmonate on biomass and anthocyanins production in cell suspension culture of Melastoma malabathricum (Melastomaceae). Rev Biol Trop 59:597–606

    PubMed  Google Scholar 

  • Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S (2009) Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 22:102–106

    CAS  PubMed  Google Scholar 

  • Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of Light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41:1176–1185

    Google Scholar 

  • Tariq U, Ali M, Abbasi BH (2014) Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. J Photochem Photobiol B: Biol 130:264–271

    Article  CAS  Google Scholar 

  • Wind J, Smeekens S, Hanson J (2010) Sucrose: metabolite and signaling molecule. Phytochemistry 71:1610–1614

    Article  CAS  PubMed  Google Scholar 

  • Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199

    Article  CAS  Google Scholar 

  • Wu CH, Murthy HN, Hahn EJ, Paek KY (2007) Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactor for the production of chichoric acid, chlorogenic acid and cafraric acid. Biotechnol Lett 29:1179–1182

    Article  CAS  PubMed  Google Scholar 

  • Zhang YH, Zhong JJ, Yu JT (1996) Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: manipulation of medium sucrose. J Biotechnol 51:49–56

    Article  CAS  Google Scholar 

  • Zhong JJ, Seki T, Kinoshita S, Yoshida T (1991) Effect of light irradiation on anthocyanin production by suspended culture of Perilla frutescens. Biotech Bioeng 38:653–658

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Hina Fazal

  2. Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, 25120, Pakistan

    Hina Fazal

  3. Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Bilal Haider Abbasi & Mohammad Ali

  4. Center for Biotechnology and Microbiology, University of Swat, Swat, 19200, Pakistan

    Nisar Ahmad & Shahid Ali

Authors
  1. Hina Fazal
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  2. Bilal Haider Abbasi
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  3. Nisar Ahmad
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  4. Mohammad Ali
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  5. Shahid Ali
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Corresponding author

Correspondence to Bilal Haider Abbasi.

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Fazal, H., Abbasi, B.H., Ahmad, N. et al. Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L.. Plant Cell Tiss Organ Cult 124, 573–581 (2016). https://doi.org/10.1007/s11240-015-0915-z

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  • DOI: https://doi.org/10.1007/s11240-015-0915-z

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