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In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum

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Biologia Plantarum

Abstract

The effect of various hormonal combinations on callus formation and regeneration of shoot and root from leaf derived callus of Acanthophyllum sordidum Bunge ex Boiss. has been studied. Proteins and activity of antioxidant enzymes were also evaluated during shoot and root organogenesis from callus. Calli were induced from leaf explants excised from 30-d-old seedlings grown on Murashige and Skoog medium containing 4.52 µM 2,4-dichlorophenoxyacetic acid + 4.65 µM kinetin. Maximum growth of calli and the most efficient regeneration of shoots and roots occurred with 2.69 µM 1-naphthalene acetic acid (NAA), 2.69 µM NAA + 4.54 µM thidiazuron and 2.46 µM indole-3-butyric acid. Protein content decreased in calli and increased significantly during regeneration of shoots from callus. Superoxide dismutase activity decreased in calli comparing to that of seedlings, then increased in regenerated shoots and roots. High catalase activity was detected in seedlings and regenerated shoots, whereas high peroxidase activity was observed in calli and regenerated roots.

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Abbreviations

MS:

Murashige and Skoog

2,4-D:

2,4-dichlorophenoxy acetic acid

Kin:

kinetin

NAA:

1-naphthalene acetic acid

IBA:

indole-3-butyric acid

TDZ:

thidiazuron

BA:

benzyl adenine

IAA:

indole-3-acetic acid

SOD:

superoxide dismutase

CAT:

catalase

POX:

peroxidase

ROS:

reactive oxygen species

SDS:

sodium dodesyl sulfate

PAGE:

polyacrylamide gel electrophoresis

Zea:

zeatin

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, 13145, I.R. Iran

    A. A. Meratan & S. M. Ghaffari

  2. Department of Plant Sciences, School of Biology, University of Tehran, Tehran, 14155, I.R. Iran

    V. Niknam

Authors
  1. A. A. Meratan
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  2. S. M. Ghaffari
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  3. V. Niknam
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Correspondence to V. Niknam.

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Meratan, A.A., Ghaffari, S.M. & Niknam, V. In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum . Biol Plant 53, 5–10 (2009). https://doi.org/10.1007/s10535-009-0002-6

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  • DOI: https://doi.org/10.1007/s10535-009-0002-6

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