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Free Radical Scavenging Activity of Aqueous and Ethanolic Extract of Brassica oleracea L. var. italica

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Abstract

In this study, antioxidant activities of aqueous and ethanolic extracts of Brassica oleracea L. var. italica were investigated. The antioxidant properties of both extracts of Brassica oleracea L. var. italica were evaluated using different antioxidant tests, including 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide radical scavenging, inhibition of microsomal lipid peroxidation, reduction of power, and metal ion chelating activities. Inhibition of superoxide scavenging by aqueous and ethanolic extracts showed an IC50 of 0.93 and 0.25 mg/ml, respectively. Metal ion chelation showed an IC50 of 0.35 mg/ml of both the extracts and was equipotent to positive control, ethylenediamine tetra-acetic acid. The ethanolic extract of Brassica oleracea L. var. italica exhibited higher antioxidant activity in DPPH radical and superoxide anion scavenging than that of aqueous extract. The results obtained in the in vitro models clearly suggest that, Brassica oleracea L. var. italica is a natural source for antioxidants, which could serve as a nutraceutical with potential applications in reducing the level of oxidative stress and related health benefits. However, comprehensive studies need to be conducted to ascertain the in vivo safety of such extracts in experimental animal models.

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Acknowledgements

The authors wish to thank Prof. C.K. Renukarya, Director of the Institute for his keen interest in this study. Thanks to Dr. Jyothi Bala Chauhan, Reader, Department of Biotechnology, Mahajana PG Center, Mysore, for her support.

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  1. Department of Studies in Biotechnology, Pooja Bhagavat Memorial Mahajana Post Graduate Center, Wing of SBRR Mahajana Education Centre, Mysore, 570016, Karnataka, India

    Abdul Mueed Bidchol, A. Wilfred, P. Abhijna & R. Harish

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  1. Abdul Mueed Bidchol
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Correspondence to R. Harish.

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Bidchol, A.M., Wilfred, A., Abhijna, P. et al. Free Radical Scavenging Activity of Aqueous and Ethanolic Extract of Brassica oleracea L. var. italica . Food Bioprocess Technol 4, 1137–1143 (2011). https://doi.org/10.1007/s11947-009-0196-9

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