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Accumulation and tolerance of Cr and Pb using a cell suspension culture system of Jatropha curcas

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Abstract

Jatropha curcas has shown to be a bioaccumulator and a tolerant species to different heavy metals (HMs). This study presents an evaluation system for tolerance and accumulation of chromium (Cr) and lead (Pb) in J. curcas using a cell suspension culture (CSC). This CSC was established with callus obtained from hypocotyls segments, onto Murashige-Skoog medium and 2.27 µM 2,4-dichlorophenoxyacetic acid (2,4-D). CSC growth kinetics showed a specific cell growth rate (µ) of 0.092 d−1 and a doubling time of 7.5 d. Exposure of the CSC to HMs (Cr, or Pb at 0.0, 0.5, 1.0, 2.0 and 3.0 mM), resulted in a significant effect (P ≤ 0.05) on biomass accumulation. By adding Cr (0.5 or 1.0 mM) or any Pb concentration, biomass was increased. Maximum biomass production (15.1 g L−1) was shown with Pb (3.0 mM), while all tolerance index values were between 47.8 and 136 %. In addition, the maximum accumulation of Cr (6,368 mg kg−1) and Pb (8,608 mg kg−1) were found by adding the highest concentrations used of Cr or Pb (3 mM). Bioaccumulation factors (BF) were higher (29 and 41) in the high concentrations of 2.0 and 3.0 mM Cr, respectively. In contrast, maximum BF values of Pb (16.6 and 19.4) were observed when low concentrations of Pb were used (1.0 and 0.5 mM, respectively). This is the first study reporting HMs accumulation and tolerance in J. curcas CSC. Therefore, in further studies, this culture system may be used as a model to explore the cellular mechanisms used for tolerance and accumulation of HMs.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BF:

Bioaccumulation factor

Cd:

Cadmium

Cr:

Chromium

CSC:

Cell suspension culture

Cu:

Copper

DW:

Dry weight

FW:

Fresh weight

HDPE:

High density polyethylene flasks

HMs:

Heavy metals

MS:

Murashige and Skoog (1968) medium

Pb:

Lead

PGRs:

Plant growth regulators

Dt:

Doubling time

TI:

Tolerance index

µ:

Growth specific rate

Zn:

Zinc

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Acknowledgments

The study reported here was carried out during a postdoctoral stay supported by the Consejo Nacional de Ciencia y Tecnología (CONACYT) (project 99963: 2012-2014). We are grateful to Ricardo Cruz Huizache for his kind support in Laboratory assistance and Atomic Absorption Spectrometer procedures.

Conflict of interest

The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca, Morelos, Mexico

    A. Bernabé-Antonio & L. Álvarez

  2. Facultad de Ciencias, Universidad Autónoma del Estado de México, Campus El Cerrillo, Piedras Blancas, Carretera Toluca-Ixtlahuaca km. 15.5, C.P. 50200, Toluca, Estado de México, Mexico

    L. Buendía-González

  3. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, C. P. 09340, México, D.F, Mexico

    A. Maldonado-Magaña & F. Cruz-Sosa

Authors
  1. A. Bernabé-Antonio
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  2. L. Álvarez
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  3. L. Buendía-González
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  4. A. Maldonado-Magaña
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  5. F. Cruz-Sosa
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Corresponding author

Correspondence to F. Cruz-Sosa.

Additional information

Authors A. Bernabé-Antonio, L. Álvarez, L. Buendía-González, A. Maldonado-Magaña, F. Cruz-Sosa have contributed equally to this work.

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Bernabé-Antonio, A., Álvarez, L., Buendía-González, L. et al. Accumulation and tolerance of Cr and Pb using a cell suspension culture system of Jatropha curcas . Plant Cell Tiss Organ Cult 120, 221–228 (2015). https://doi.org/10.1007/s11240-014-0597-y

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