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Overexpression of PeHSF mediates leaf ROS homeostasis in transgenic tobacco lines grown under salt stress conditions

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Abstract

Transcriptional responses of a heat-shock transcription factor from the salt-resistant Populus euphratica, PeHSF, were assessed in transgenic tobacco seedlings subjected to salinity stress. Moreover, their relevance to NaCl-induced anti-oxidative defense responses in higher plants was also explored. Expression of PeHSF was induced by high-salinity (150 mM NaCl) in leaves and callus cultures of P. euphratica. The salt-stimulated PeHSF expression in P. euphratica callus cultures was markedly inhibited by DPI (an inhibitor of plasma membrane NADPH oxidase) and LaCl3 (an inhibitor of plasma membrane Ca2+-permeable channels), indicating the involvement of reactive oxygen species (ROS) and Ca2+ in HSF activation under NaCl stress. Sequence analysis showed that PeHSF is a typical class A HSF in higher plants. PeHSF overexpression in tobacco significantly improved the salt tolerance of transgenic plants, in term of seed germination and root growth. Compared to the wild-type plants, the PeHSF-transgenic lines exhibited an increased capacity to control ROS homeostasis, but not an enhanced capacity to retain ionic homeostasis under salt stress. PeHSF expression in tobacco enhanced the activities of ascorbate peroxidase, glutathione peroxidase, and glutathione reductase, due to upregulated transcriptional levels. We conclude that PeHSF plays a crucial role in ROS detoxification under salt stress, rather than in maintenance of the K+/Na+. It is likely that the salt-induced H2O2 and cytosolic Ca2+ led to transcriptional activation of HSF, which initiated the transcription of genes encoding antioxidant enzymes in P. euphratica, thus contributing to ROS homeostasis control under saline conditions.

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Acknowledgments

This research was supported jointly by the National Natural Science Foundation of China (Grant Nos. 31270654, 31170570, and 31200470), the Key Grant Project of the Chinese Ministry of Education (Grant No. 2013001), the Beijing Natural Science Foundation (Grant No. 6112017), the Program of Introducing Talents of Discipline to Universities (111 Project, Grant No. B13007), Fundamental Research Funds for the Central Universities (Grant Nos. JC2011-2 and TD-2012-04), the Foundation for the Supervisors of Beijing Excellent Doctoral Dissertations (Grant No. YB20081002201).

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  1. College of Biological Sciences and Technology, Beijing Forestry University, Box 162, Beijing, 100083, People’s Republic of China

    Zedan Shen, Mingquan Ding, Jian Sun, Shurong Deng, Rui Zhao, Meijuan Wang, Xujun Ma, Feifei Wang, Huilong Zhang, Zeyong Qian, Yue Hu, Rui Yu, Xin Shen & Shaoliang Chen

  2. College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou, 311300, Zhejiang Province, People’s Republic of China

    Mingquan Ding

  3. College of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu Province, People’s Republic of China

    Jian Sun

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  1. Zedan Shen
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Correspondence to Shaoliang Chen.

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Shen, Z., Ding, M., Sun, J. et al. Overexpression of PeHSF mediates leaf ROS homeostasis in transgenic tobacco lines grown under salt stress conditions. Plant Cell Tiss Organ Cult 115, 299–308 (2013). https://doi.org/10.1007/s11240-013-0362-7

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