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Direct shoot bud organogenesis and plant regeneration from pre-plasmolysed leaf explants in Catharanthus roseus

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Abstract

A protocol for high-frequency shoot bud regeneration from the leaves of Catharanthus roseus is reported here for the first time. A 60-min pre-plasmolytic treatment of leaf explants in a cell protoplast washing medium containing 13% (w/v) mannitol followed by their plating on a half-strength Murashige and Skoog (MS) medium supplemented with 7.0 mg/l 6-benzyladenine (BA) and 3.0 mg/l α-naphthaleneacetic acid (NAA) resulted in the de novo induction and development of adventitious shoot buds in more than 75% of explants. Histological observations revealed a direct origin of these shoot buds from hypodermal tissue around the mid-rib. The rooting in the regenerated shoots was obtained in the presence of 3.0 mg/l indole-3-butyric acid (IBA) and the rooted plants could be successfully established in soil with a 70% rate of success. The relevance of the developed protocol in terpenoid indole alkaloids pathway engineering at the whole-plant level in C. roseus is discussed.

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Acknowledgments

This work was financially supported by the Council of Scientific and Industrial Research (CSIR), New Delhi, India, through its Network Project COR-02. The help rendered by our colleague Dr. G.D. Bagchi during the micro-photography is immensely acknowledged. P.V. also thanks the CSIR for awarding a senior research fellowship to her during the course of this investigation. We are also grateful to the Director of the Central Institute of Medicinal and Aromatic Plants (CIMAP) for providing the facilities and support.

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  1. Plant Tissue Culture Laboratory, Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, PO CIMAP, Kukrail Picnic Spot Road, Lucknow, 226015, India

    Priyanka Verma & Ajay Kumar Mathur

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  1. Priyanka Verma
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  2. Ajay Kumar Mathur
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Correspondence to Ajay Kumar Mathur.

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Verma, P., Mathur, A.K. Direct shoot bud organogenesis and plant regeneration from pre-plasmolysed leaf explants in Catharanthus roseus . Plant Cell Tiss Organ Cult 106, 401–408 (2011). https://doi.org/10.1007/s11240-011-9936-4

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  • DOI: https://doi.org/10.1007/s11240-011-9936-4

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