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Induction and origin of adventitious shoots from chimeras of Brassica juncea and Brassica oleracea

  • Cell Biology and Morphogenesis
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Abstract

The chimeras between tuber mustard (Brassica juncea) and red cabbage (B. oleracea) were artificially synthesized in our previous study. Adventitious shoots were induced from nodal segments and leaf discs of TCC (LI-LII-LIII, LI -the outmost layer of shoot apical meristem; LII -the middle layer; LIII -the innermost layer. T = Tuber mustard, C = Red cabbage) chimeras. The origin of the shoots was analyzed by histology and molecular biology. As a result, the frequency of adventitious shoot induction rose with the increase of BA in MS medium in the area of the nodes. However, there was no different induction frequency of adventitious shoots from nodal segment bases in media with different BA concentrations. Most adventitious shoots (clustered shoots) arising from the node area were TTT (Tuber mustard- Tuber mustard- Tuber mustard) and only 4 shoots were chimeras, which indicated that more shoots originated from LI than from LII and LIII. All shoots from nodal segment bases were CCC (Red cabbage-Red cabbage- Red cabbage), indicating that the shoots originated from LII or LII and LIII. There were significant differences in the regeneration rate in the margin of the leaf discs among the three combinations of BA and NAA. Most adventitious shoots from the margin of leaf discs were CCC but 2 out of 70 were chimeras, which indicated that more shoots originated from LII or LII and LIII than from LI. All chimeras obtained by regeneration were different from the original explant donor in type in the present study. The origin of the adventitious shoots varied with the site of origin on the donor plant, and could be multicellular and multihistogenic.

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Abbreviations

BA:

6-Benzyladenine acid

NAA:

α-Naphthaleneacetic acid

MS:

Murashige and Skoog medium

LMIAS:

Leaf margin-induced adventitious shoots

LVIAS:

Leaf vein-induced adventitious shoots

NBIAS:

Nodal base-induced adventitious shoots

NIAS:

Nodal-induced adventitious shoots

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 30671427).

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Authors and Affiliations

  1. Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Xue-Yun Zhu, Man Zhao, Sheng Ma, Ya-Ming Ge, Ming-Fang Zhang & Li-Ping Chen

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  1. Xue-Yun Zhu
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  2. Man Zhao
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  3. Sheng Ma
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  4. Ya-Ming Ge
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  5. Ming-Fang Zhang
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  6. Li-Ping Chen
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Correspondence to Li-Ping Chen.

Additional information

Communicated by W. Harwood.

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Zhu, XY., Zhao, M., Ma, S. et al. Induction and origin of adventitious shoots from chimeras of Brassica juncea and Brassica oleracea . Plant Cell Rep 26, 1727–1732 (2007). https://doi.org/10.1007/s00299-007-0398-4

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  • DOI: https://doi.org/10.1007/s00299-007-0398-4

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