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Reverse-cleft in vitro micrografting of Ziziphus jujuba Mill. Infected with jujube witches’ broom (JWB)

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Abstract

Jujube witches’ broom (JWB) is a destructive disease, caused by a phytoplasma, of Chinese jujube (Ziziphus jujuba Mill.), an important traditional fruit in China and South Korea. Micrografting of plantlets to detect those affected with JWB is difficult due to differences in stem diameter between the scion and rootstock. In this study, an in vitro micrografting technique, reverse-cleft graft, suitable for rootstock thinner than scion was developed and optimized. The JWB phytoplasma was successfully transmitted into the scion from diseased rootstock using this technique as determined by polymerase chain reaction. Micrografted plantlets of Chinese jujube were incubated on Murashige and Skoog medium without plant growth regulators. The survival frequencies of 78–91%, depending on grafting combinations, were obtained.

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Abbreviations

6-BA:

6-benzyladenine

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

NAA :

α-Naphthalene acetic acid

Nested-PCR:

Nested polymerase chain reaction

PCR:

Polymerase chain reaction

PGRs:

Plant growth regulators

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Acknowledgments

This research was supported by National Key Technology R&D Program of China (2008BAD92B03), Provincial Key Technology R&D Program of Hebei (04220111D, 09230601D), and Science Fund for Distinguished Young Scholars of Hebei Province (C2010000679). The authors are grateful to Dr. Jin Zhao, Dr. Zhihui Zhao, Dr. Tabea Weihmann and Dr. Oliver Dong for critical reading of the manuscript.

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Authors and Affiliations

  1. Research Centre of Chinese Jujube, Agricultural University of Hebei, Baoding, 071001, China

    Xiaoguang Liu & Mengjun Liu

  2. Vocation College of Political Science and Law of Hebei, Shijiazhuang, 050061, China

    Qiang Ning

  3. College of Life Science, Hebei University, Baoding, 071001, China

    Guannan Liu

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  1. Xiaoguang Liu
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  2. Mengjun Liu
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  3. Qiang Ning
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  4. Guannan Liu
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Correspondence to Mengjun Liu.

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Liu, X., Liu, M., Ning, Q. et al. Reverse-cleft in vitro micrografting of Ziziphus jujuba Mill. Infected with jujube witches’ broom (JWB). Plant Cell Tiss Organ Cult 108, 339–344 (2012). https://doi.org/10.1007/s11240-011-0031-7

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