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Factors influencing efficiency of shoot regeneration in Ziziphus jujuba Mill. ‘Huizao’

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Abstract

Several factors affecting the frequency of leaf regeneration of Ziziphus jujuba ‘Huizao’ were investigated in this study, including basal medium, plant growth regulators, leaf maturity, explant orientation, and additive chemicals. The results showed that the highest shoot regeneration frequency (82.25%) was obtained on woody plant medium supplemented with 2.27 μM thidiazuron, 1.07 μM α-naphthalene-acetic acid (NAA) and 2.94 μM silver nitrate after a 10 days incubation in darkness. This study also suggested an increased regeneration frequency of expanded young leaves which were taken from the mid-stem position, as well as from the explant abaxial surface contacting with the medium. In addition, hyperhydric of adventitious buds could be effectively reduced by adding 40 g l−1 sucrose to the medium. A 95.56% rooting rate could be produced from shoots cultured on half-strength Murashige and Skoog (in Physiol Plant 15:473–497, 1962) medium plus 2.69 μM NAA.

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Fig. 1

Abbreviations

AB:

Abaxial side

AD:

Adaxial side

AgNO3 :

Silver nitrate

6-BA:

6-Benzylaminopurine

GA3 :

Gibberellic acid

IAA:

Indoleacetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

α-Naphthalene-acetic acid

PGRs:

Plant growth regulators

TDZ:

Thidiazuron

WPM:

Woody plant medium

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Acknowledgments

This research was supported by the project of Department of Science and Technology of Henan. We wish to acknowledge the assistance of Dr. Paul Burns and Dr. Xia Ye for their careful reading and critical comment on the manuscript.

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Authors and Affiliations

  1. College of Horticulture, Henan Agricultural University, 95 Wenhua Road, 450002, Zhengzhou, People’s Republic of China

    Jian-Can Feng, X. M. Yu, X. L. Shang, J. D. Li & Y. X. Wu

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  1. Jian-Can Feng
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  2. X. M. Yu
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  3. X. L. Shang
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Correspondence to Jian-Can Feng.

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Feng, JC., Yu, X.M., Shang, X.L. et al. Factors influencing efficiency of shoot regeneration in Ziziphus jujuba Mill. ‘Huizao’. Plant Cell Tiss Organ Cult 101, 111–117 (2010). https://doi.org/10.1007/s11240-009-9663-2

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