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Influence of various carbohydrates in shoot development in nodal culture of Guinean Anacardium occidentale genotypes

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Abstract

The influence of various carbohydrates in shoot development was studied in single nodal culture of Guinean cashew genotypes. Both apical and axillary nodal sections from one-and-half-year-old stock plants of elite selected cashews from Guinea-Bissau were used as explants. Six types of carbohydrates (sucrose, maltose, glucose, fructose, galactose and sorbitol) were tested separately at concentration of 83 mM. Sucrose, maltose and fructose showed the best performance and were additionally tested at concentrations of 0, 83 and 167 mM in order to evaluate the optimal concentration that promotes growth. These results indicated that the inclusion of a carbohydrate source is essential for shoot development. In the tested conditions the concentration of 83 mM was sufficient for shoot development since no significant differences were found when explants were cultured on a higher concentration. Moreover, the results showed that in spite of the concentration it is the type of carbohydrate that influences the shoot response. Maltose increased the number of developed shoots whereas fructose enhanced shoot length. These results suggested that a combination of these two carbohydrates could increase the yield of well-developed shoots in a single step. The effect of maltose and fructose was analysed separately and combined. The combination of fructose with maltose (each at 83 mM) promoted both the highest percentage of developed shoots and the highest shoot length when compared with the results obtained using these two sugars separately. With this study, we found a one-step carbohydrate combination that allowed overcoming the low yield of well-developed shoots observed in the current propagation system in Guinean cashew genotypes.

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Abbreviations

2iP:

2-isopentenyladenine

DS:

developed shoots

FR:

fructose

GA:

galactose

GA3 :

gibberellic acid

GL:

glucose

MA:

maltose

MS:

Murashige and Skoog (1962) medium

SID:

shoots that initiated development

SL:

shoot length

SO:

sorbitol

SU:

sucrose

References

  • Ananthakrishnan G, Ravikumar R, Girija S, Ganapathi A, (2002) In vitro adventitious shoot formation cotyledon explants of cashew Anacardium occidentale L.Sci. Hortic. 93: 343–355

    Article  Google Scholar 

  • Ananthakrishnan G, Ravikumar R, Prem Anand R, Vengadesan G, Ganapathi A, (1999) Induction of somatic embryogenesis from nucellus-derived callus of Anacardium occidentale L.Sci. Hortic. 79: 91–99

    Article  Google Scholar 

  • Bessa AMS, Tambá-Bungué P & Sardinha RMA (1996) Bases para o melhoramento do cajueiro (Anacardium occidentale L.) na Guiné-Bissau, situação actual e estratégia de investigação. In: Instituto de Investigação Científica e Tropical (ed) Actas do Simpósio Agricultura e Agro-Indústria Tropicais - Estudos, Empreendimentos e Cooperação, Lisbon

  • Blanc G, Lardet L, Martin A, Jacob JL, Carron MP, (2002) Differential carbohydrate metabolism conducts morphogenesis in embryogenic callus of Hevea brasiliensis (Mull. Arg.) J. Exp. Bot. 53: 1453–1462

    Article  PubMed  CAS  Google Scholar 

  • Boggetti B, Jasik J, Mantell S, (1999) In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plantsPlant Cell Rep. 18: 456–461

    Article  CAS  Google Scholar 

  • Boggetti B, Jasik J, Mantell SH, (2001) In vitro root formation in Anacardium occidentale microshootsBiol. Plant. 44: 175–179

    Article  CAS  Google Scholar 

  • Borkowska B, Szczerba J, (1991) Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures J. Exp. Bot. 42: 911–915

    Article  CAS  Google Scholar 

  • Brown DCW, Leung DWM, Thorpe TA, (1979) Osmotic requirement for shoot formation in tobacco callusPhysiol. Plant. 46: 36–41

    Article  CAS  Google Scholar 

  • D’Silva I, D’Souza L, (1992) In vitro propagation of Anacardium occidentale LPlant Cell Tiss. Org. Cult. 29: 1–6

    Article  CAS  Google Scholar 

  • Gibson SI, (2003) Sugar and phytohormone response pathways: navigating a signalling networkJ. Exp. Bot. 55: 253–264

    Article  PubMed  Google Scholar 

  • Iraqi D, Tremblay FM, (2001) Analysis of carbohydrate metabolism enzymes and cellular contents of sugars and proteins during spruce somatic embryogenesis suggests a regulatory role of exogenous sucrose in embryo developmentJ. Exp. Bot. 52: 2301–2311

    Article  PubMed  CAS  Google Scholar 

  • Lipavská H, Konrádová H, (2004) Somatic embryogenesis in conifers: the role of carbohydrate metabolismIn vitro Cell. Dev. Biol. Plant 40: 23–30

    Article  Google Scholar 

  • Mantell SH, Boggetti B, Bessa AMS, Lemos EP, Abdelhadi A & Mneney EE (1998) Micropropagation and micrografting methods suitable for international transfers of cashew. In: Topper CP, Caligari PDS, Kullaya AK, Shomani SH, Kasuga LJ, Masawe PA & Mpunami AA (eds) Proceedings of the International Cashew and Coconut Conference, Dar Es Salaam (pp 128–133). BioHybrids International Ltd., Reading

  • Maurel K, Sakr S, Gerbe F, Guilliot A, Bonhomme M, Rageau R, Pétel G, (2004) Sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree budsJ. Exp. Bot. 55: 879–888

    Article  PubMed  CAS  Google Scholar 

  • Mchouachi J, Tadeo FR, Zaragosa S, Primo-Millo E, Talon M, (1996) Effects of gibberellic acid and paclobutrazol on growth and carbohydrate accumulation in shoots and roots of citrus rootstock seedlingsJ. Hort. Sci. 71: 747–754

    Google Scholar 

  • Mneney EE, Mantell SH, (2001) In vitro micrografting of cashewPlant Cell Tiss. Org. Cult. 66: 49–58

    Article  Google Scholar 

  • Mneney EE, Mantell SH, (2002) Clonal propagation of cashew (Anacardium occidentale L.) by tissue cultureJ. Hortic. Sci. Biotech. 77: 649 –657

    CAS  Google Scholar 

  • Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue culturesPhysiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Sardinha RMA, Bessa AMS, Blake J, Guyer D, Cassam C & Tamba-Bungue P (1993) Selection of superior genotypes of cashew (Anacardium occidentale L.) in Guinea-Bissau and the development of in vitro techniques for their propagation. Final Scientific Report EU Contract N°TS2-A-0167-P

  • Schuller A, Reuther G, (1993) Response of Abies alba embryonal-suspensor mass to various carbohydrate treatmentsPlant. Cell Rep. 12: 199–202

    Article  CAS  Google Scholar 

  • Scott P, Lyne RL, Rees T, (1995) Metabolism of maltose and sucrose by microspores isolated from barley (Hordeum vulgare L.)Planta 197: 435–441

    Article  CAS  Google Scholar 

  • Sheen J, Zhou L, Jang JC, (1999) Sugars as signalling moleculesCurr. Opin. Plant. Biol. 2: 410–418

    Article  PubMed  CAS  Google Scholar 

  • Slesak H, Skoczowski A, Pryzywara L, (2004) Exogenous carbohydrate utilisation by explants of Brassica napus cultured in vitroPlant Cell Tiss. Org. Cult. 79: 45–51

    Article  CAS  Google Scholar 

  • Smeekens S, (2000) Sugar-induced signal transduction in plantsAnnu. Rev. Plant. Physiol. Plant. Mol. Biol. 51: 49–81

    Article  PubMed  CAS  Google Scholar 

  • StaSoft (1995). Statistica for Windows™ (Vol. I and III). StatSoft, Inc, USA

    Google Scholar 

  • Taber RP, Zhang C, Hu WS, (1998) Kinetics of Douglas-fir (Pseudotsuga menziesii) somatic embryo developmentCan. J. Bot. 76: 863–871

    Article  CAS  Google Scholar 

  • Tang GQ, Luscher M, Sturm A, (1999) Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose partitioningPlant Cell 11: 177–189

    Article  PubMed  CAS  Google Scholar 

  • Thimmappaiah, Puthra GT, Shirly RA, (2002a) In vitro grafting of cashew (Anacardium occidentale L.)Sci. Hortic. 92: 177–182

    Article  Google Scholar 

  • Thimmappaiah, Shirly RA, Sadhana PH, (2002b) In vitro propagation of cashew from young trees In vitro Cell Dev. Biol. Plant 38: 152–156

    Article  Google Scholar 

  • Thorpe TA, (1974) Carbohydrate availability in shoot formation in tobacco callus culturesPhysiol. Plant. 30: 77–81

    Article  CAS  Google Scholar 

  • Tremblay L, Tremblay FM, (1991) Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) BSP) and red spruce (P. rubens Sarg.) somatic embryosPlant Cell Tiss. Org. Cult. 27: 95–103

    Article  CAS  Google Scholar 

  • Welander M, Welander NT, Brackman AS, (1989) Regulation of in vitro shoot multiplication in Syringa, Alnus and Malus by different carbon sourcesJ. Hortic. Sci. 64: 361–366

    CAS  Google Scholar 

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Acknowledgements

This project was supported by the European Union contract n° TS3-CP93-0221. The author Gemas had an MSc grant from PRAXIS XXI (BM/8174/96). The authors are grateful to Professor Pedro Fevereiro and Drs Susana Araújo and Sofia Duque from the Plant Cell Biotechnology Laboratory of the Instituto de Tecnologia Química e Biológica, for their helpful comments and the critical reading of the manuscript and to Mrs Paula Alves and Mr Paulo Tambá for their technical assistance.

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Authors and Affiliations

  1. Instituto de Tecnologia Química e Biológica, Plant Cell Biotechnology Laboratory, 6th floor, Apt. 127, 2781-901, Oeiras, Portugal

    Vitória Gemas

  2. Instituto de Investigação Científica Tropical, Quinta do Marquês, 2780-157, Oeiras, Portugal

    Ana Bessa

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  1. Vitória Gemas
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  2. Ana Bessa
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Correspondence to Vitória Gemas.

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Gemas, V., Bessa, A. Influence of various carbohydrates in shoot development in nodal culture of Guinean Anacardium occidentale genotypes. Plant Cell Tiss Organ Cult 85, 103–108 (2006). https://doi.org/10.1007/s11240-005-9051-5

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