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Introgression of Recombinant 1RSWR.1BL Translocation and Rust Resistance Genes in Bread Wheat cv. HD2967 Through Marker-Assisted Selection

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Abstract

Bread wheat (Triticum aestivum L.) is one of the major cereal crops utilized worldwide for bread making. The presence of secalin locus on 1RS leads to the sticky dough and poor bread-making quality of wheat. In the present study, two donor parents, one with distal rye chromatin (1RS44:38) and another with distal wheat chromatin (Pavon MA1) without secalin, and one recipient elite wheat cultivar HD2967 were used. In 1RS44:38, the distal rye region has the Pm8 gene to which the QTL for superior root traits is linked, while in Pavon MA1 with Glu-B3/Gli-B1, the Pm8 gene was found to be absent. This distal rye region having root trait QTL was introgressed into the HD2967 derivatives using marker-assisted backcross selection. The derivatives with distal rye region introgression had higher root biomass, drought resistance, and 6–8% higher yield than the recipient parent cultivar. HD2967 is highly susceptible to yellow rust. Therefore, in the second backcross, the rust-resistant version of HD2967 (Lr57 + Yr40) was used to introgress rust resistance in the derivatives. Background selection was done using polymorphic wheat anchored SSR markers of A, B, and D genomes of wheat which led to the selection of derivatives with > 90% background of the recipient cultivar. The significant findings in this study include higher root biomass, improved yield, rust resistance in the derivatives, and retaining the alleles of Glu-B3/Gli-B1 along with Pm8 and the absence of secalin.

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Acknowledgements

The authors acknowledge Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, for providing necessary infrastructure and research facilities for carrying out this work.

Funding

The authors received financial support from the Department of Biotechnology, Government of India (BT/PR10886/AGII/106/934/2014).

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Authors and Affiliations

  1. Department of Genetics-Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, 173101, H.P., India

    Ambika Sharma, Imran Sheikh, Pritesh Vyas & H. S. Dhaliwal

  2. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141027, Punjab, India

    Satinder Kaur

Authors
  1. Ambika Sharma
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  2. Imran Sheikh
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  3. Satinder Kaur
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  4. Pritesh Vyas
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  5. H. S. Dhaliwal
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Correspondence to Pritesh Vyas or H. S. Dhaliwal.

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Key Message

• The introgression of the major QTL linked to Pm8 from the distal 1RS region led to the higher root biomass in the elite wheat cultivar derivatives. The introgression also led to the higher yield and drought tolerance in the derivatives. The recombination between Pm8 and Glu-B3/Gli-B1 was achieved in the absence of secalin.

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Sharma, A., Sheikh, I., Kaur, S. et al. Introgression of Recombinant 1RSWR.1BL Translocation and Rust Resistance Genes in Bread Wheat cv. HD2967 Through Marker-Assisted Selection. Plant Mol Biol Rep 39, 841–849 (2021). https://doi.org/10.1007/s11105-021-01295-6

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