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Identification and introgression of a novel leaf rust resistance gene from Thinopyrum intermedium chromosome 7Js into wheat

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Abstract

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A novel leaf rust resistance locus located on a terminal segment (0–69.29 Mb) of Thinopyrum intermedium chromosome arm 7JsS has been introduced into wheat genome for disease resistance breeding.

Abstract

Xiaoyan 78829, a wheat–Thinopyrum intermedium partial amphiploid, exhibits excellent resistance to fungal diseases in wheat. To transfer its disease resistance to common wheat (Triticum aestivum), we previously developed a translocation line WTT26 using chromosome engineering. Disease evaluation showed that WTT26 was nearly immune to 14 common races of leaf rust pathogen (Puccinia triticina) and highly resistant to Ug99 race PTKST of stem rust pathogen (P. graminis f. sp. tritici) at the seedling stage. It also displayed high adult plant resistance to powdery mildew (caused by Blumeria graminis f. sp. tritici). Cytogenetic and molecular marker analysis revealed that WTT26 carried a T4BS·7JsS chromosome translocation. Once transferred into the susceptible wheat genetic background, chromosome 7JsS exhibited its resistance to leaf rust, indicating that the resistance locus was located on this alien chromosome. To enhance the usefulness of this locus in wheat breeding, we further developed several new translocation lines with small Th. intermedium segments using irradiation and developed 124 specific markers using specific-locus amplified fragment sequencing, which increased the marker density of chromosome 7JsS. Furthermore, a refined physical map of chromosome 7JsS was constructed with 74 specific markers, and six bins were thus arranged according to the co-occurrence of markers and alien chromosome segments. Combining data from specific marker amplification and resistance evaluation, we mapped a new leaf rust resistance locus in the 0–69.29 Mb region on chromosome 7JsS. The translocation lines carrying the new leaf rust resistance locus and its linked markers will contribute to wheat disease-resistance breeding.

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Data availability

Phenotype data of agronomic traits are presented in Table 2. The genotype data and materials reported in this study are available upon request.

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Acknowledgements

We sincerely thank Prof. Wenxiang Yang at the Department of Plant Pathology, Hebei Agricultural University, for providing help with leaf rust assessment.

Funding

This project was supported by the National Natural Science Foundation of China (No. 31971875) and the National Key Research and Development Program of China (2016YFD0102000).

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Guotang Yang, Hongwei Li, Bin Li, Zhensheng Li & Qi Zheng

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Guotang Yang

  3. Department of Plant Pathology, Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, Baoding, 071001, Hebei, China

    Na Zhang

  4. Department of Plant Sciences, University of the Free State, Bloemfontein, 9300, South Africa

    Willem H. P. Boshoff

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  1. Guotang Yang
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  2. Na Zhang
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  3. Willem H. P. Boshoff
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  4. Hongwei Li
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  5. Bin Li
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Contributions

ZSL and QZ conceived the research; GTY performed the experiments; NZ performed leaf rust resistance evaluation; WB performed stem rust resistance evaluation; GTY and QZ drafted the manuscript; HWL and BL provided substantial help in preparing materials. All authors read and approved the final manuscript.

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Correspondence to Qi Zheng.

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Yang, G., Zhang, N., Boshoff, W.H.P. et al. Identification and introgression of a novel leaf rust resistance gene from Thinopyrum intermedium chromosome 7Js into wheat. Theor Appl Genet 136, 231 (2023). https://doi.org/10.1007/s00122-023-04474-z

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