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Downregulation of OsPK1 Contributes to Oxidative Stress and the Variations in ABA/GA Balance in Rice

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Abstract

Abscisic acid (ABA) is a phytohormone that aids plants in coping with stress conditions. ABA and gibberellin (GA) are hormone partners that function via a complicated and antagonistic network. In ospk1, a dwarf rice mutant, the contents of ABA in the youngest leaf sheaths of 6-week-old seedlings and the uppermost internodes of heading stage plants were both increased, and the synthesis of bioactive GAs was suppressed, which may disharmonize ABA/GA balance. In ospk1, expression of three putative enzyme genes related to stress response was upregulated. A strong browning symptom was observed in the second internode (Int2, counted from the top) and part of panicles of ospk1 at the late productive phase. Furthermore, higher levels of H2O2 in flag leaf and Int2 were observed in ospk1 than those in wild type. These data suggest that ospk1 may undergo certain stress, especially oxidative stress. Here, we provide evidences that the downregulation of OsPK1 (a cytosolic pyruvate kinase) in ospk1 mutant results in variations in ABA/GA balance in rice and contributes to oxidative stress, which provide a new clue for understanding the connection of pyruvate kinase, ABA/GA balance, and oxidative stress in rice.

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Acknowledgments

We thank Xiangning Jiang and Shichang Liu of Beijing Forestry University for technical assistance in measurement of phytohormones. This work was supported by the Natural Science Foundation of Hainan Province (grant no. 30204).

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Authors and Affiliations

  1. School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Yan Zhang & Feng Feng

  2. Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China

    Yan Zhang, Feng Feng & Chaozu He

  3. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, Haikou, Hainan, 570228, China

    Chaozu He

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  1. Yan Zhang
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Correspondence to Chaozu He.

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Zhang, Y., Feng, F. & He, C. Downregulation of OsPK1 Contributes to Oxidative Stress and the Variations in ABA/GA Balance in Rice. Plant Mol Biol Rep 30, 1006–1013 (2012). https://doi.org/10.1007/s11105-011-0386-2

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