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Glucohexaose-induced protein phosphatase 2C regulates cell redox status of cucumber seedling

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Abstract

Protein Phosphatase 2C (PP2C) is an important phosphatase-like protein in eukaryotic organisms that can negatively regulate protein kinase cascade abscisic acid (ABA) signal system through phosphorylation and carry out vital roles in various cell processes. The previous study indicated that the accumulation of reactive oxygen species (ROS) is a part of mechanism of glucohexaose-induced resistance in cucumber cotyledons, and CsPP2C80s might play a crucial role in processes related to ROS produce and signal transduction. To identify the mechanism of CsPP2C80s involved in glucohexaose and ABA signaling regulating cell redox status, the effects of glucohexaose and ROS inhibitor pretreatment on endogenous ABA content and ABA signaling genes expression levels of cucumber seedlings were analysed. These results suggest that cucumber CsPP2C80s are involved in ROS accumulation and ABA signal transduction pathway induced by glucohexaose, CsPP2C80s play a positive regulatory role in process of ABA combined with ABA receptors (PYLs) to activate SNF1-related protein kinases 2 (SnRK2s) and regulate NADPH oxidase to produce extracellular hydrogen peroxide (H2O2), providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in cell redox status induced by glucohexaose.

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Acknowledgements

This work was supported by the Program for Excellent Researcher in University of Liaoning Province (LR2014019) and the Talents Engineering of Liaoning Province Project (2014921040). All the authors are thankful to Prof. J Ning for providing glucohexaose.

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Authors and Affiliations

  1. College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Q M Chen, N Cui & H Y Fan

  2. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Y Yu, C M Lin & H Y Fan

  3. Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    N Cui & T F Song

  4. Liaoning Academy of Agricultural Sciences, Shenyang, 110866, People’s Republic of China

    J Y Zhao

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  1. Q M Chen
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  2. Y Yu
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  3. C M Lin
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  4. N Cui
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  5. J Y Zhao
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  6. T F Song
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  7. H Y Fan
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Correspondence to N Cui or H Y Fan.

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Corresponding editor: Agepati S Raghavendra

Corresponding editor: Agepati S Raghavendra

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Chen, Q.M., Yu, Y., Lin, C.M. et al. Glucohexaose-induced protein phosphatase 2C regulates cell redox status of cucumber seedling. J Biosci 43, 117–126 (2018). https://doi.org/10.1007/s12038-018-9738-5

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  • DOI: https://doi.org/10.1007/s12038-018-9738-5

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