Root nutrient concentration and biomass allocation are more plastic than morphological traits in response to nutrient limitation

Abstract

Background and aims

Understanding the magnitude of phenotypic plasticity within a species is important, particularly when comparing species from diverse habitats or when using traits from global databases. Our objective was to quantify the magnitude of intraspecific variability of fine root, stem, and leaf traits in response to nutrient availability.

Methods

We measured growth rates and traits from fine roots, stems, and leaves on replicate seedlings of four species grown in two treatments: low and high soil nutrient availability. We used ANOVA to test for effects of fertilizer, species, and their interaction on trait expression, and ranked intraspecific trait variation using the coefficient of variation.

Results

Root nutrient concentration, relative growth rate, and biomass allocation exhibited the most plastic responses to nutrient availability. Specific root length, specific leaf area, root diameter, and wood density were the least variable traits within species. Nutrient limitation only induced greater root branching in the non-mycorrhizal Proteaceae species that produced more cluster roots.

Conclusions

Woody plants respond to nutrient limitation by increasing root mass fraction, rather than by adjusting root morphology or structure. We urge caution when using root tissue chemistry traits obtained from global databases in local studies, as this is one of the most plastic traits in response to nutrient availability.

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Abbreviations

RGR:

Relative growth rate

SLA:

Specific leaf area

SRL:

Specific root length

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Acknowledgements

This research was supported by a grant (UOW1201) from the Royal Society of New Zealand Marsden Fund, and a University of Waikato Research Scholarship. We thank Toni Cornes, Lynne Baxter, Adam Purcell, and Angela Simpson for their assistance in this study.

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Correspondence to Kris R. Kramer-Walter or Daniel C. Laughlin.

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Kramer-Walter, K.R., Laughlin, D.C. Root nutrient concentration and biomass allocation are more plastic than morphological traits in response to nutrient limitation. Plant Soil 416, 539–550 (2017). https://doi.org/10.1007/s11104-017-3234-9

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Keywords

  • Plant traits
  • Specific root length
  • Specific leaf area
  • Biomass allocation
  • Soil fertility
  • Phenotypic plasticity
  • Nutrient availability
  • Intraspecific trait variation