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Hydrogen enhances adaptation of rice seedlings to cold stress via the reestablishment of redox homeostasis mediated by miRNA expression

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Abstract

Aims

The role of hydrogen gas (H2) in modulating cold tolerance was investigated.

Methods

A combination of physiological and molecular approaches was used to study the effect of H2 on the alleviation of cold stress.

Results

Cold stress stimulated the production of H2 in rice seedlings. The pretreatment with exogenous H2 not only mimicked above physiological response, but also prevented cold-induced growth inhibition. With 0.39 mM H2 pretreatment, rice seedlings exhibited the maximum improving responses, including alleviating the decrease of chlorophyll contents and photosynthetic activity. The lipid peroxidation and the overproduction of reactive oxygen species (ROS) were decreased as well. These results were supported by the changes of total and isozymatic activities of antioxidant enzymes. In comparison with cold stress alone, miR398 transcripts were down-regulated by H2, and expression levels of its targets Cu/Zn-SOD1 (CSD1) and Cu/Zn-SOD2 (CSD2) were increased. By contrast, miR319 transcripts were differentially increased, showing a relatively negative correlation with its target genes PROLIFERATING CELL FACTOR5 (PCF5) and PROLIFERATING CELL FACTOR8 (PCF8).

Conclusions

Our results suggested that endogenous H2 might contribute to the enhancement of cold tolerance, at least partially, by the reestablishment of redox homeostasis via miR398 and miR319.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

Ci:

Intercellular CO2 concentration

CSD :

Cu/Zn-SOD

DAB:

3, 3′-diaminobenzidine

GC:

Gas chromatography

Gs:

Stomatal conductance

H2 :

Hydrogen gas

miRNA:

MicroRNA

NBT:

Nitroblue tetrazolium

PCF:

Proliferating Cell Factor

Pn:

Net photosynthetic rate

POD:

Guaiacol peroxidase

ROS:

Reactive oxygen species

RWC:

Relative water content

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid reactive substances

TCP:

TEOSINTE BRANCHED/CYCLOIDEA/PCF

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Acknowledgments

This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Natural Science Foundation of Jiangsu Province (BK20130683), the Fundamental Research Funds for the Central Universities (KYTZ201402), and the National Natural Science Foundation of China (J1210056 and J1310015).

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Authors and Affiliations

  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China

    Sheng Xu, Duo Bu, Jiangyan Fu & Ren Wang

  2. College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Yilong Jiang, Weiti Cui, Qijiang Jin, Yihua Zhang, Duo Bu & Wenbiao Shen

  3. Shenzhen Kelieng Biomedical Technology Co., Ltd, Shenzhen, 518000, China

    Feng Zhou

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  1. Sheng Xu
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Correspondence to Wenbiao Shen.

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Xu, S., Jiang, Y., Cui, W. et al. Hydrogen enhances adaptation of rice seedlings to cold stress via the reestablishment of redox homeostasis mediated by miRNA expression. Plant Soil 414, 53–67 (2017). https://doi.org/10.1007/s11104-016-3106-8

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