Abstract
Aims
This study investigated the molecular mechanism of hydrogen-rich water (HRW)-mediated enhancement of tolerance against salinity stress during rice seed germination.
Methods
A combination of physiological and molecular approaches was used to study the effect of HRW on the alleviation of salinity stress.
Results
A 100-mM NaCl stress caused the increase of H2 release in germinating rice seeds. With respect to samples treated with 100 mM NaCl alone, exogenous HRW pretreatments differentially attenuated the inhibition of seed germination and seedling growth caused by salinity. Further results showed that both 50 % (in particular) and 100 % concentration of HRW could activate α/β-amylase activity, thus accelerating the formation of reducing sugar and total soluble sugar. HRW also enhanced total, isozymatic activities or corresponding transcripts of antioxidant enzymes, including superoxide dismutase, catalase, and ascorbate peroxidase. These results were confirmed by the alleviation of oxidative damage, as indicated by a decrease of thiobarbituric acid reactive substances. Additionally, the ratio of potassium (K) to sodium (Na) in both the shoot and root parts was increased.
Conclusions
Together, our results suggested that exogenous HRW treatment on rice seeds may be a good option to alleviate salinity stress.
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Abbreviations
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- DHAR:
-
Dehydroascorbate reductase
- GR:
-
Glutathione reductase
- HRW:
-
Hydrogen-rich water
- MDHAR:
-
Monodehydroascorbate reductase
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- TBARS:
-
Thiobarbituric acid reactive substances
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Acknowledgments
This work was supported by the Key Science and Technology Specific Projects of Guizhou Province (grant no. 2012-6005), the Rice Breeding, Cultivation and Industrialization Program of Guizhou Province (grant no. 20114003), and the Jiangsu Agricultural Science and Technology Independence Innovation Project (grant no. CX(11)4022).
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Xu, S., Zhu, S., Jiang, Y. et al. Hydrogen-rich water alleviates salt stress in rice during seed germination. Plant Soil 370, 47–57 (2013). https://doi.org/10.1007/s11104-013-1614-3
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DOI: https://doi.org/10.1007/s11104-013-1614-3