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Chlorophyll fluorescence imaging can reflect development of vascular connection in grafting union in some Solanaceae species

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Photosynthetica

Abstract

Graft union development in plants has been studied mainly by destructive methods such as histological studies. The aim of this work was to evaluate whether the chlorophyll fluorescence imaging (CFI) technique is sensitive enough to reflect changes at the cellular level in different Solanaceae grafted plants 30 d after grafting, when both grafted partners were well fused and strong enough in all plant combinations. The pepper cultivar ‘Adige’ was grafted onto different Capsicum spp. accessions typified with different compatibility degrees; eggplant was grafted on Solanum torvum and pepper homografts as compatible unions; pepper was grafted on S. torvum and on tomato as incompatible unions. ‘Adige’/’Adige’ and ‘Adige’/pepper A25 showed a higher maximum quantum efficiency of PSII associated with higher values of actual quantum efficiency of PSII and photochemical quenching as well as with vascular regeneration across the graft interface. Our results highlighted that CFI changes reflected histological observations in grafted Solanaceae plants.

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Abbreviations

A:

Capsicum annuum L. var. Adige

A25:

C. annuum accession A25

B14:

C. baccatum accession B14

C12:

C. chinense accession C12

BEU:

tomato rootstock Beufort

CFI:

chlorophyll fluorescence imaging

Chl:

chlorophyll

DAG:

days after grafting

EGG:

eggplant var. Cristal

Fm :

maximal fluorescence yield of the dark-adapted samples

Fm`:

maximal fluorescence yield of the light-adapted samples

Fo :

minimal fluorescence yield of the dark-adapted samples

Fo`:

minimal fluorescence yield of the light-adapted samples

Fs :

steady-state fluorescence yield during actinic irradiation

Fv :

variable fluorescence (Fm–Fo) in the dark-adapted samples

Fv/Fm :

maximum quantum efficiency of PSII photochemistry

NPQ:

nonphotochemical quenching calculated from Stern-Volmer equation

qP :

photochemical quenching

ST:

Solanum torvum

TOM:

tomato var. Gordal

ΦPSII :

actual quantum efficiency of PSII

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Authors and Affiliations

  1. Departamento de Horticultura, Instituto Valenciano de Investigaciones Agrarias (IVIA)., Valencia, Spain

    C. Penella & Ángeles Calatayud

  2. Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza, Spain

    A. Pina

  3. Departamento de Producción Vegetal, Universitat Politècnica de València., Valencia, Spain

    A. San Bautista & S. López-Galarza

Authors
  1. C. Penella
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  2. A. Pina
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  3. A. San Bautista
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  4. S. López-Galarza
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  5. Ángeles Calatayud
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Correspondence to Ángeles Calatayud.

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Acknowledgements: This work was financed by INIA (Spain) through Project RTA2013-00022-C02-01 and the European Regional Development Fund (ERDF).

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Penella, C., Pina, A., San Bautista, A. et al. Chlorophyll fluorescence imaging can reflect development of vascular connection in grafting union in some Solanaceae species. Photosynthetica 55, 671–678 (2017). https://doi.org/10.1007/s11099-017-0690-7

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  • DOI: https://doi.org/10.1007/s11099-017-0690-7

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