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Role of Grafting in Resistance to Water Stress in Tomato Plants: Ammonia Production and Assimilation

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Abstract

In general, drought depresses nutrient uptake by the root and transport to the shoot due to a restricted transpiration rate, which may contribute to growth limitation under water deprivation. Moreover, water stress may also restrict the ability of plants to reduce and assimilate nitrogen through the inhibition of enzymes implicated in nitrogen metabolism. The assimilation of nitrogen has marked effects on plant productivity, biomass, and crop yield, and nitrogen deficiency leads to a decrease in structural components. Plants produce significant quantities of NH4 + through the reduction of NO3 − and photorespiration, which must be rapidly assimilated into nontoxic organic nitrogen compounds. The aim of the present work was to determine the response of reciprocal grafts made between one tomato tolerant cultivar (Lycopersicon esculentum), Zarina, and a more sensitive cultivar, Josefina, to nitrogen reduction and ammonium assimilation under water stress conditions. Our results show that when cv. Zarina (tolerant cultivar) was used as rootstock grafted with cv. Josefina (ZarxJos), these plants showed an improved N uptake and NO3 − assimilation, triggering a favorable physiological and growth response to water stress. On the other hand, when Zarina was used as the scion (JosxZar), these grafted plants showed an increase in the photorespiration cycle, which may generate amino acids and proteins and could explain their better growth under stress conditions. In conclusion, grafting improves N uptake or photorespiration, and increases leaf NO3 − photoassimilation in water stress experiments in tomato plants.

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Acknowledgments

This work was financed by the PAI program (Plan Andaluz de Investigación, Grupo de Investigación AGR161) and by a Grant from the FPU of the Ministerio de Educación y Ciencia awarded to ESR.

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Sciences, University of Granada, 18071, Granada, Spain

    Eva Sánchez-Rodríguez, Luis Romero & Juan Manuel Ruiz

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  1. Eva Sánchez-Rodríguez
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  2. Luis Romero
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  3. Juan Manuel Ruiz
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Correspondence to Eva Sánchez-Rodríguez.

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Sánchez-Rodríguez, E., Romero, L. & Ruiz, J.M. Role of Grafting in Resistance to Water Stress in Tomato Plants: Ammonia Production and Assimilation. J Plant Growth Regul 32, 831–842 (2013). https://doi.org/10.1007/s00344-013-9348-2

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  • DOI: https://doi.org/10.1007/s00344-013-9348-2

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