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Investigation of Drug Delivery by Iontophoresis in a Surgical Wound Utilizing Microdialysis

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Abstract

Purpose

This study investigated the penetration of lidocaine around and through a sutured incision following the application of iontophoretic and passive patches in the CD Hairless rat.

Materials and Methods

Concentrations in localized areas (suture, dermis, subcutaneous, and vascular) were determined using microdialysis sampling followed by analysis using liquid chromatography with UV detection.

Results

Iontophoresis significantly enhanced the dermal penetration of lidocaine. In an intact skin model, dermal concentrations were 40 times greater following iontophoretic delivery compared to passive delivery. In a sutured incision model, iontophoresis enhanced localized concentrations in the dermis, suture, and subcutaneous regions by 6-, 15-, and 20-fold, respectively. Iontophoretic delivery to a region containing a sutured incision was focused to the incision resulting in a greater increase in the suture concentration and in the subcutaneous region directly below the incision.

Conclusions

The four microdialysis probe design was successful in the determination of localized drug penetration in a sutured incision model. Iontophoresis enhanced skin penetration and allowed for site specific delivery when applied to a sutured incision.

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Acknowledgements

The authors would like to thank the Lawrence Memorial Hospital, especially Dr. Mike Thompson, for their help with processing the histology slides. This work was funded by Travanti Pharma, Inc.

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Authors and Affiliations

  1. R. N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, Kansas, 66047, USA

    Heidi J. Holovics & Craig E. Lunte

  2. Travanti Pharma Inc., 2520 Pilot Knob Road Suite 100, Mendota Heights, Minnesota, 55128, USA

    Carter R. Anderson

  3. Hospira, Inc., 275 North Field Drive, Lake Forest, Illinois, 60045, USA

    Barry S. Levine & Ho-Wah Hui

Authors
  1. Heidi J. Holovics
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  2. Carter R. Anderson
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  3. Barry S. Levine
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  4. Ho-Wah Hui
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  5. Craig E. Lunte
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Corresponding author

Correspondence to Craig E. Lunte.

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Holovics, H.J., Anderson, C.R., Levine, B.S. et al. Investigation of Drug Delivery by Iontophoresis in a Surgical Wound Utilizing Microdialysis. Pharm Res 25, 1762–1770 (2008). https://doi.org/10.1007/s11095-007-9490-2

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  • DOI: https://doi.org/10.1007/s11095-007-9490-2

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