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Analysis of Eleutherosides by Tandem Mass Spectrometry: Possibilities of Standardizing a Multi-Phytoadaptogen Formulation for Preventive Oncology

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Pharmaceutical Chemistry Journal Aims and scope

The major bioactive constituents in extracts from roots of Eleutherococcus senticosus (Rupr. & Maxim) (Araliaceae) and in a multi-adaptogen herbal formulation (Multiphytoadaptogen) were analyzed using high-performance liquid chromatography (HPLC) in combination with tandem mass spectrometry. Chromatography was performed on an ACQUITY UPLC BEH C18 column in gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used for the analysis. Eleutherosides B (syringin, a phenylpropanoid) and E (syringaresinol diglucoside, a lignan) were identified in both the multi-adaptogen herbal formulation and E. senticosus root extract. The results could be used for standardization and quality testing of herbal formulations including eleutherosides B and E and for justification of the biological action of Multiphytoadaptogen and studies of its new properties considering the identified bioactive constituents. The probable mechanisms of the antitumor and additive/synergistic effects of eleutherosides B and E were established by in silico analysis of their biological activity profiles.

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Acknowledgments

The work was performed with partial support by grants from the Commission on Biomedical Innovations and Technologies, Ministry of Science of the Russian Federation and in the framework of the Basic Research Program in the Russian Federation for the Long Term (2021 – 2030) (No. 122030100170-5).

CONFLICT OF INTEREST

We confirm no conflicts of interest.

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Authors and Affiliations

  1. N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, 24 Kashirskoe Shosse, Moscow, 115478, Russia

    O. A. Bocharova, V. E. Shevchenko, I. V. Kazeev, E. V. Bocharov, R. V. Karpova, A. A. Aksenov & V. S. Kosorukov

  2. Scientific Center for Biomedical Technologies, All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), 7/1 Grina St, Moscow, 117216, Russia

    O. P. Sheichenko

  3. V. N. Orekhovich Institute of Biomedical Chemistry, 10/8 Pogodinskaya St, Moscow, 119121, Russia

    N. S. Ionov & V. V. Poroikov

  4. Research Institute of General Pathology and Pathophysiology, 8 Baltiiskaya St, Moscow, 125315, Russia

    V. G. Kucheryanu

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  1. O. A. Bocharova
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Correspondence to I. V. Kazeev.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 56, No. 6, pp. 29 – 37, June, 2022.

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Bocharova, O.A., Shevchenko, V.E., Kazeev, I.V. et al. Analysis of Eleutherosides by Tandem Mass Spectrometry: Possibilities of Standardizing a Multi-Phytoadaptogen Formulation for Preventive Oncology. Pharm Chem J 56, 806–814 (2022). https://doi.org/10.1007/s11094-022-02712-0

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