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Histopathological vascular investigation of the peritumoral brain zone of glioblastomas

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Abstract

To date, no histopathological vascular investigation focusing on peritumoral brain zone (PBZ) has been reported for glioblastoma. We analyzed 10 newly diagnosed cases of glioblastomas. For these PBZs, histopathological investigation was performed by hematoxylin–eosin (H&E) staining and immunohistochemistry was analyzed for CD31, CD34, Factor VIII, VEGF, VEGFR-1/2, Ki67, p53 and nestin. Although it was difficult to identify PBZ by H&E, Ki67 and p53 staining, there were apparent differences in nestin staining among PBZ, tumor core (TC), and normal zone (NZ). Therefore, in this study, we divided PBZ from TC and NZ by nestin staining. Differences in histological features, microvessel density, expression of VEGF and its receptors were assessed for PBZ, TC and NZ. The microvessel density, as determined by counting CD31, CD34 and VEGF receptors, and VEGF-A expression were lower in PBZ than TC. The expression patterns for CD31, CD34 and VEGF receptors in vessels show dissociation in PBZ. In addition, the vascular characteristics of the PBZ may correlate with findings of radiographic imaging. We provide the first clinicopathological evidence that PBZ exhibits unique angiogenic characteristics. These in situ observations will help to elucidate the mechanisms of tumor recurrence.

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Acknowledgements

The authors greatly thank Ms. Naoko Tsuzaki and Tomoko Muraki at Department of Neurosurgery, Keio University School of Medicine, for technical assistance of laboratory works.

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Authors and Affiliations

  1. Department of Neurosurgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Ryota Tamura, Hikaru Sasaki, Yukina Morimoto, Kazunari Yoshida & Masahiro Toda

  2. Department of Pathology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Kentaro Ohara

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  1. Ryota Tamura
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Correspondence to Masahiro Toda.

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Tamura, R., Ohara, K., Sasaki, H. et al. Histopathological vascular investigation of the peritumoral brain zone of glioblastomas. J Neurooncol 136, 233–241 (2018). https://doi.org/10.1007/s11060-017-2648-9

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