Abstract
There is a general agreement on the Nestin re-expression in reactive astrocytes, but its modalities differ among experimental animal species and between the latter and human material. In a series of 40 surgical specimens, including gliomas, vascular malformations, abscesses and angiomas, the glial reaction has been studied by immunohistochemistry and immunofluorescence of Nestin, GFAP and Vimentin. The observations made by immunohistochemistry were comparable with those by immunofluorescence. In some lesions, glial reaction was long-lasting and astrocytes were in the same late maturation stage. In other lesions, such as invading malignant gliomas, astrocytes occurred in different maturation stages. In comparison with GFAP, Nestin was poorly expressed in mature astrocytes and more expressed in developing reactive astrocytes, mainly in the cytoplasms, with a great variability, and much less in the processes. In the invading tumor, developing positive astrocytes were hardly distinguishable from tumor invading astrocytes that, interestingly, were much more Nestin- than GFAP-positive. In the deep tumor reactive astrocytes were no more visible. The interpretation of the findings was based on what is known on the reciprocal behavior of the three antigens in maturing astrocytes during embryogenesis and on the hypothesis of an embryonic regression of reactive astrocytes. The impossibility to distinguish them from tumor cells in the deep tumor legitimates the suspicion of their recruitment among tumor cells.
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References
Herrmann H, Aebi U (2004) Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular scaffolds. Annu Rev Biochem 73:749–789
Pixley SKT, De Vellis J (1984) Transition between immature radial glia and mature astrocytes studied with a monoclonal antibody to vimentin. Dev Brain Res 317:201–209
Hernandez MR, Agapova GA, Yang P, Salvador-Silva M, Ricard CS, Aoi S (2002) Differential gene expression in astrocytes from human normal and glaucomatous optic nerve head analyzed by cDNA microarray. Glia 38:45–64
Buschong EA, Martone ME, Ellisman MH (2004) Maturation of astrocyte morphology and the establishment of astrocyte domain during postnatal hippocampal development. Int J Dev Neurosci 22: 73–86
Clarke SR, Shetty AK, Bradely JL, Turner DA (1994) Reactive astrocytes express the embryonic intermediate protein nestin. NeuroReport 5:1885–1888
Eliasson C, Sahlgren C, Berthold CH et.al (1999) Intermediate filament protein partnership in astrocytes. J Biol Chem 274:23996–24006
Wei LC, Shi M, Chen LW, Cao R, Zhang P, Chan YS (2002) Nestin containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of post-natal developing and adult mice. Dev Brain Res 139:9–17
Duggal N, Schmidt-Kastner R, Hakim AM (1997) Nestin expression in reactive astrocytes following focal cerebral ischemia in rat. Brain Res 768:1–9
Schmidt-Kastner R, Garcia I, Busto R, Ginsberg MD (1997) Nestin antibodies label reactive glial cells and some cortical neurons after global brain ischemia in adult rat. J Cerebr Blood Flow Metabol 17(suppl 1):727
Sahin Kaya S, Mahmood A, Li Y, Yavuz E, ChoppM (1999) Expression of nestin after traumatic brain injury in the rat. Brain Res 840:153–157
Takamiya Y, Kohsaka S, Toy S, Otani M, Tsukada Y (1988) Immunohistoichemical studies on the proliferation of reactive astrocytes and the expression of cytoskeletal proteins following brain injury in rats. Dev Brain Res 38:201–210
Schiffer D, Giordana MT, Cavalla P, Vigliani MC, Attanasio A (1993) Immunohistochemistry of glial reaction after injury in the rat: double staining and markers of cell proliferation. Int J Devl Neurosci 11:269–280
Frisén J, Johansson CB, Töröet C, Risling M, Lendhal U (1995) Rapid, widespread, and long-lasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. The J Cell Biol 131:453–464
Holmin S, Almqvist P, Landhal U, Mathiesen T (1997) Adult nestin-expressing subependymal cells differentiate to astrocytes in response to brain injury. Eur J Neurosci 119:65–75
Schiffer D (1997) Brain tumors. Biology, pathology and clinical references, 2nd edn. Springer, Berlin-Heidelberg-New York
Abdel-Rahman, Rao MS, Shetty AK, (2004) Nestin expression in hippocampal astrocytes after injury depends on the age of the hippocampus. Glia 47:299–313
Geloso C, Corvino V, Cavallo V, Toesca A, Guadagni E, Passalacqua R, Michetti F (2004) Expression of astrocytic nestin in the rat hippocampus during trimethyltin-induced neurodegeneration. Nerosci Lett 357:103–106
Lin RCS, Matesic DF, Marvin M, McKay RDG, Brustle O (1995) Reexpression of the intermediate filament nestin in reactive astrocytes. Neurobiol Dis 2:79–85
Li Y, Chopp M (1999) Temporal profile of nestin expression after focal cerebral ischemia in adult rat. Brain Res 838:1–10
Krum JM, Phillips TM, Rosenstein JM (2002) Changes in astroglial GLT–1 expression after neural transplantation ir stab wounds. Exp Neurol 174:137–149
Holley JE, Gveric D, Newcombe J, Cuznert ML, Gutowski NJ (2003) Astrocytes characterization in the multiple sclerosis glial scar. Neuropathol Appl Neurobiol 29:434–444
Dahlstrand, Collins VP, Lendahl U (1992) Expression of the class VI intermediate filament Nestin in human central nervous system tumors. Cancer Res 52:5334–5341
Almqvist M, Mah R, Lendahl U, Jacobsson B, Hendson G (2002) Immunohistochemical detection of Nestin in pediatric brain tumors. J Histocytochem Cytochem 50:147–158
Krum JM, Rosenstein JM (1999) Transient coexpression of nestin, GFAP and vascular endothelial growth factor in mature reactive astrocytes following neural grafting of brain wounds. Exp Neurol 160:348–360
Wu VW, Schwartz JP (1998) Cell culture models for reactive gliosis : new perspectives. J Neurosci Res 51:675–681
Schiffer D., Manazza A., Tamagno I. Nestin expression in neuroepithelial tumors. Neurosi Lett 2006 [in press]
Fomchenko EI, Holland EC (2005) Stem cells and brain cancer. Exp Cell Res 306:323–329
Shih AH, Holland EC (2004) Developmental neurobiology and the origin of brain tumors. J Neuro-oncol 70:125–136
Doetsch F (2003) The glia identity for neural stem cell. Nature Neurosci 6:1127–1134
Rutka JT, Ivanchuk S, Mondal S, Taylor M, Sakai K, Dirks P, Jun P, Becker LE, Ackerley C (1999) Co-expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells. Int J Dev Neuroscience 17:503–515
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Tamagno, I., Schiffer, D. Nestin expression in reactive astrocytes of human pathology. J Neurooncol 80, 227–233 (2006). https://doi.org/10.1007/s11060-006-9181-6
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DOI: https://doi.org/10.1007/s11060-006-9181-6