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Review: role of carbon sources for in vitro plant growth and development

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Abstract

In vitro plant cells, tissues and organ cultures are not fully autotrophic establishing a need for carbohydrates in culture media to maintain the osmotic potential, as well as to serve as energy and carbon sources for developmental processes including shoot proliferation, root induction as well as emission, embryogenesis and organogenesis, which are highly energy demanding developmental processes in plant biology. A variety of carbon sources (both reducing and non-reducing) are used in culture media depending upon genotypes and specific stages of growth. However, sucrose is most widely used as a major transport-sugar in the phloem sap of many plants. In micropropagation systems, morphogenetic potential of plant tissues can greatly be manipulated by varying type and concentration of carbon sources. The present article reviews the past and current findings on carbon sources and their sustainable utilization for in vitro plant tissue culture to achieve better growth rate and development.

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Horticulture, PMAS-Arid Agriculture University, Rawalpindi, Pakistan

    Mehwish Yaseen, Touqeer Ahmad & Ishfaq Ahmad Hafiz

  2. Sustainable Agro-ecosystems and Bioresources Department, IASMA Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all’Adige, (TN), Italy

    Gaurav Sablok

  3. Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Perugia, Perugia, PG, Italy

    Alvaro Standardi

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  1. Mehwish Yaseen
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  2. Touqeer Ahmad
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  5. Ishfaq Ahmad Hafiz
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Correspondence to Ishfaq Ahmad Hafiz.

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Yaseen, M., Ahmad, T., Sablok, G. et al. Review: role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40, 2837–2849 (2013). https://doi.org/10.1007/s11033-012-2299-z

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