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Requirements for in vitro propagation of seven nitrogen-fixing Alnus species

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Abstract

Studies on the in vitro propagation of Alnus crispa, A. glutinosa, A. incana, A. japonica, A. rubra, A. sinuata and A. viridis indicated interspecific as well as intraspecific variations in their requirements for in vitro culture. The WPM and Blaydes media supported, respectively, growth of A. glutinosa and A. crispa but not that of both species, while the MS medium induced equal or significantly better growth than WPM and Blaydes media for both species. The optimum type and concentration of sugar to be used in the multiplication medium varied with species. Only A. glutinosa showed good growth on sucrose while glucose was optimum for all other species but at different concentrations. All species rooted in 3 weeks on half-strength MS medium including 1 μM IBA. All clones of A. glutinosa and A. rubra rooted 100%, whereas “easy-to-root” and “difficult-to-root” clones were observed in the other species. In the rooting medium, glucose promoted rooting of the “difficult-to-root” clones better than sucrose. Survival following transfer to an artificial substrate was 100% for all species. Nodulation tests using pure cultures of two Frankia strains showed 100% nodulation on all Alnus clones.

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Authors and Affiliations

  1. Faculté de Foresterie, Dépt Sciences Forestières, Université Laval, G1K 7P4, Ste-Foy, Québec, Canada

    Francine M. Tremblay & Maurice Lalonde

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  1. Francine M. Tremblay
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  2. Maurice Lalonde
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Tremblay, F.M., Lalonde, M. Requirements for in vitro propagation of seven nitrogen-fixing Alnus species. Plant Cell Tiss Organ Cult 3, 189–199 (1984). https://doi.org/10.1007/BF00040337

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  • DOI: https://doi.org/10.1007/BF00040337

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