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Molecular characterization of oilseed rape accessions collected from multi continents for exploitation of potential heterotic group through SSR markers

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Abstract

Evaluation of the genetic diversity in conventional and modern rapeseed cultivars is essential for conservation, management and utilization of these genetic resources for high yielding hybrid production. The objective of this research was to evaluate a collection of 86 oilseed rape cultivars with 188 simple sequence repeat (SSR) markers to assess the genetic variability, heterotic group identity and relationships within and between the groups identified among the genotypes. A total of 631 alleles at 188 SSR markers were detected including 53 and 84 unique and private alleles respectively, which indicated great richness and uniqueness of genetic variation in these selected cultivars. The mean number of alleles per locus was 3.3 and the average polymorphic information content was 0.35 for all microsatellite loci. Unweighted Pair Group Method with Arithmetic Mean clustering and principal component analysis consistently divided all the cultivars into four distinct groups (I, II, III and IV) which largely coincided with their geographical distributions. The Chinese origin cultivars are predominantly assembled in Group II and showed wide genetic base because of its high allelic abundance at SSR loci while most of the exotic cultivars grouped into Group I and were highly distinct owing to the abundant private and unique alleles. The highest genetic distance was found between Group I and IV, which mainly comprised of exotic and newly synthesized yellow seeded (1728-1 and G1087) breeding lines, respectively. Our study provides important insights into further utilization of exotic Brassica napus accessions in Chinese rapeseed breeding and vice versa.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (No. 31071452) and the Doctoral Fund of Ministry of Education of China (No. 20100146110019).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Muhammad Younas, Yingjie Xiao, Dongfang Cai, Wei Yang, Wei Ye, Jiangsheng Wu & Kede Liu

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  1. Muhammad Younas
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  2. Yingjie Xiao
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  4. Wei Yang
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  7. Kede Liu
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Correspondence to Kede Liu.

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M. Younas and Y. Xiao contributed equally to this work.

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Younas, M., Xiao, Y., Cai, D. et al. Molecular characterization of oilseed rape accessions collected from multi continents for exploitation of potential heterotic group through SSR markers. Mol Biol Rep 39, 5105–5113 (2012). https://doi.org/10.1007/s11033-011-1306-0

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