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Microsatellite characterization of Andean races of common bean (Phaseolus vulgaris L.)

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Abstract

The Andean gene pool of common bean (Phaseolus vulgaris L.) has high levels of morphological diversity in terms of seed color and size, growth habit and agro-ecological adaptation, but previously was characterized by low levels of molecular marker diversity. Three races have been described within the Andean gene pool: Chile, Nueva Granada and Peru. The objective of this study was to characterize a collection of 123 genotypes representing Andean bean diversity with 33 microsatellite markers that have been useful for characterizing race structure in common beans. The genotypes were from both the primary center of origin as well as secondary centers of diversity to which Andean beans spread and represented all three races of the gene pool. In addition we evaluated a collection of landraces from Colombia to determine if the Nueva Granada and Peru races could be distinguished in genotypes from the northern range of the primary center. Multiple correspondence analyses of the Andean race representatives identified two predominant groups corresponding to the Nueva Granada and Peru races. Some of the Chile race representatives formed a separate group but several that had been defined previously as from this race grouped with the other races. Gene flow was more notable between Nueva Granada and Peru races than between these races and the Chile race. Among the Colombian genotypes, the Nueva Granada and Peru races were identified and introgression between these two races was especially notable. The genetic diversity within the Colombian genotypes was high, reaffirming the importance of this region as an important source of germplasm. Results of this study suggest that the morphological classification of all climbing beans as Peru race genotypes and all bush beans as Nueva Granada race genotypes is erroneous and that growth habit traits have been mixed in both races, requiring a re-adjustment in the concept of morphological races in Andean beans.

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References

  • Afanador L, Hadley S, Kelly JD (1993) Adoption of a mini-prep DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.). Bean Improv Coop 36:10–11

    Google Scholar 

  • Becerra V, Gepts P (1994) RFLP diversity of common bean (Phaseolus vulgaris) in its centres of origin. Genome 37:256–263

    Article  Google Scholar 

  • Beebe S, Toro O, González AV, Chacón MI, Debouck D (1997) Wild-weed-crop complex of common bean (Phaseolus vulgaris L., Fabaceae) in the Andes of Peru and Colombia, and their implications for conservation and breeding. Genet Resour Crop Evol 44:73–91

    Article  Google Scholar 

  • Beebe S, Skroch P, Tohme J, Duque MC, Pedraza F, Nienhuis J (2000) Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Sci 40:264–273

    Article  Google Scholar 

  • Beebe S, Renjifo J, Gaitán-Solís E, Duque MC, Tohme J (2001) Diversity and origin of Andean landraces of common bean. Crop Sci 41:854–862

    Article  Google Scholar 

  • Beer S, Siripoonwiwat W, O’Donoughue L, Souza E, Matthews D, Sorrells M (1997) Associations between molecular markers and quantitative traits in an oat germplasm pool: can we infer linkages? J Agric Genomics 3:1–16

    Google Scholar 

  • Blair MW, Pedraza F, Buendia H, Gaitan E, Beebe S, Gepts P, Tohme J (2003) Development of a genome wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374

    Article  PubMed  CAS  Google Scholar 

  • Blair MW, Giraldo MC, Buendia HF, Tovar E, Duque MC, Beebe S (2006) Microsatellite marker diversity in common bean (Phaseolus vulgaris L.). Theor Appl Genet 113:100–109

    Article  PubMed  CAS  Google Scholar 

  • Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Bean (Phaseolus spp.); model food legumes. Plant Soil 252:55–128

    Article  CAS  Google Scholar 

  • Chacón MI, Pickersgill S, Debouck D (2005) Domestication patterns in common bean (Phaseolus vulgaris L.) and the origin of Mesoamerican and Andean cultivated races. Theor Appl Genet 110:432–444

    Article  CAS  Google Scholar 

  • Debouck D, Toro O, Paredes O, Johnson W, Gepts P (1993) Genetic diversity and ecological distribution of Phaseolus vulgaris L. (Fabacea) in northwestern south Am Econ Bot 47:408–423

    Google Scholar 

  • Díaz LM, Blair MW (2006) Race structure within the Mesoamerican gene pool of common bean (Phaseolus vulgaris L.) as determined by microsatellite markers. Theor Appl Genet 114: 143–154

    Article  PubMed  Google Scholar 

  • Diaz LM, Diaz JM, Blair MW (2005) Diversidad genetica de frijol comun (Phaseolus vulgaris L.) en Colombia. Fitotecnia Colomb 5:28–36

    Google Scholar 

  • Duarte J, Dos Santos J, Melo L (1999) Genetic divergence among common beans cultivars from different races based on RAPD markers. Genet Mol Biol 22:419–426

    Google Scholar 

  • Durán LA, Blair MW, Giraldo MC, Machiavelli R, Prophete E, Nin JC, Beaver JS (2005) Morphological and molecular characterization of common bean (Phaseolus vulgaris L.) landraces from the Caribbean. Crop Sci 45:1320–1328

    Article  Google Scholar 

  • Gaitán E, Duque MC, Edwards K, Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris L.): isolation, characterization, and cross-species amplification in Phaseolus spp. Crop Sci 42:2128–2136

    Article  Google Scholar 

  • Gepts P, Osborn T, Rashka K, Bliss F (1986) Phaseolin–protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris L.): evidence for multiple centers of domestication. Econ Bot 40:451–468

    CAS  Google Scholar 

  • Gepts P (1988) Phaseolin as an evolutionary marker. In: Resources of Phaseolus beans. Kluwer, Dordtrecht, pp 215–241

  • Gepts P (1998) Origin and evolution of common bean: past events and recent trends. HortScience 33:1124–1130

    Google Scholar 

  • Gepts P, Bliss F (1986) Phaseolin variability among wild and cultivated common bean (Phaseolus vulgaris L.) from Colombia. Econ Bot 40:469–478

    CAS  Google Scholar 

  • Hidalgo R, Rubiano H, Toro O (1992) Common bean Phaseolus vulgaris L. germplasm catalog. Working document No. 14. ISNN 0120–6525. CIAT

  • Islam FM, Basford KE, Redden RJ, Gonzalez AV, Kroonenberg PM, Beebe SE (2002) Genetic variability in cultivated common bean beyond the two major gene pools. Genet Resour Crop Evol 49:271–283

    Article  Google Scholar 

  • Islam FM, Beebe S, Muñoz M, Tohme J, Redden RJ, Basford KE (2004) Using molecular markers to assess the effect of introgression on quantitative attributes of common bean in the Andean gene pool. Theor Appl Genet 108:243–252

    Article  PubMed  CAS  Google Scholar 

  • Johns M, Skroch P, Nienhuis J, Hinrichsen P, Bascur G, Muñoz-Schick C (1997) Gene pool classification of common bean landraces from Chile based on RAPD and Morphological data. Crop Sci 37:605–613

    Article  Google Scholar 

  • Koenig R, Gepts P (1989) Allozyme diversity in wild Phaseolus vulgaris: further evidence for two mayor centers of genetic diversity. Theor Appl Genet 78:809–817

    Article  Google Scholar 

  • Maciel F, Echeverrigaray S, Gerald L, Grazziotin F (2003) Genetic relationship and diversity among Brazilian cultivar and of common beans (Phaseolus vulgaris L.) revealed by AFLP markers. Genet Resour Crop Evol 50:887–893

    Article  CAS  Google Scholar 

  • Metais I, Hamon B, Jalouzot R, Peltier D (2002) Structure and level of genetic diversity in various bean types evidenced with microsatellite markers isolated from a genomic enriched library. Theor Appl Genet 104:1346–1352

    Article  PubMed  CAS  Google Scholar 

  • Nei M (1978) Estimation of average heterozygocity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  CAS  Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics, Columbia University press, New York

    Google Scholar 

  • Paredes O, Gepts P (1995) Extensive introgression of middle American germplasm into Chilean common bean cultivars. Genet Resour Crop Evol 42:29–41

    Article  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Rohlf F (2002) NTSYS pc. Numerical Taxonomy System Exeter Publishing, Setauket, NY

  • Rosenberg NA (2002) Distruct: a program for the graphical display of structure results. http://www.cmb.usc.edu/»noahr/distruct

  • Santalla M, Rodiño A, de Ron A (2002) Allozyme evidence supporting southwestern Europe as a secondary center of genetic diversity for the common bean. Theor Appl Genet 104:934–944

    Article  PubMed  CAS  Google Scholar 

  • Santalla M, Menendez-Sevillano M, Monteagudo A, de Ron A (2004) Genetic diversity of Argentinian common bean and its evolution during domestication. Euphytica 135:75–87

    Article  CAS  Google Scholar 

  • SAS-Institute (1996) SAS/STAT Users guide 6.11. SAS Inst, Cary

  • Singh S (1989) Patterns of variation in cultivated common bean (Phaseolus vulgaris, Fabaceae). Econ Bot 43:39–57

    Google Scholar 

  • Singh S, Gepts P, Debouck D (1991a) Races of common bean (Phaseolus vulgaris, Fabaceae). Econ Bot 45:379–396

    Google Scholar 

  • Singh S, Gutierrez A, Molina A, Urrea C, Gepts P (1991b) Genetic diversity in cultivated common bean. II. Marker-based analysis on morphological and agronomic traits. Crop Sci 31:23–29

    Article  CAS  Google Scholar 

  • Singh S, Nodari R, Gepts P (1991c) Genetic diversity in cultivated common bean. I. Allozymes. Crop Sci 31:19–23

    Article  CAS  Google Scholar 

  • Slatkin M, Barton N (1989) A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43:1349–1368

    Article  Google Scholar 

  • Sonnante G, Stockton T, Nodari R, Becerra Velasquez V, Gepts P (1994) Evolution of genetic diversity during the domestication of common bean (Phaseolus vulgaris L.). Theor Appl Genet 89:629–635

    Article  Google Scholar 

  • Tohme J, Jones P, Beebe S, Iwanaga M (1995a) The combined use of agroecological and characterization data to establish the CIAT Phaseolus vulgaris core collection, In: Hodgkin T et al (eds) Core collections of plant genetic resources. Wiley, New York

  • Tohme J, Toro O, Vargas J, Debouck D (1995b) Variability in Andean Nuña common beans (Phaseolus vulgaris, Fabaceae). Econ Bot 49:78–95

    Google Scholar 

  • Voysest O, Valencia M, Amezquita M (1994) Genetic diversity among Latin American Andean and Mesoamerican common bean cultivars. Crop Sci 34:1100–1110

    Article  Google Scholar 

  • Yeh FY, Boyle R, Ye T, Mao Z (1997) POPGENE, the user friendly shareware for population genetic analysis, version 1.31. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. Molecular Biology and Biotechnology Centre

  • Yu K, Park J, Poysa V, Gepts P (2000) Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J Hered 91:429–434

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank S. Beebe and C. de Vicente for helpful suggestions during the study. We are also grateful to D. Debouck, O. Toro and A. Hoyos of the Genetic Resource Unit and Bean Projects of CIAT for germplasm and phaseolin genotyping. This research was part of theses conducted at Univ. Nacional de Colombia and was supported by the Generation Challenge Program and CIAT funds.

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Authors and Affiliations

  1. Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia

    M. W. Blair, J. M. Díaz, L. M. Díaz & M. C. Duque

  2. Universidad Nacional de Colombia, Palmira, Colombia

    J. M. Díaz & R. Hidalgo

  3. CIAT, International Center for Tropical Agriculture, 1380 N.W. 78th Ave., Miami, FL, 33126, USA

    M. W. Blair

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  1. M. W. Blair
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  2. J. M. Díaz
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  3. R. Hidalgo
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  4. L. M. Díaz
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  5. M. C. Duque
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Correspondence to M. W. Blair.

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Communicated by I. Goldman.

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Blair, M.W., Díaz, J.M., Hidalgo, R. et al. Microsatellite characterization of Andean races of common bean (Phaseolus vulgaris L.). Theor Appl Genet 116, 29–43 (2007). https://doi.org/10.1007/s00122-007-0644-8

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  • DOI: https://doi.org/10.1007/s00122-007-0644-8

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