Abstract
Rose fragrances play an important role in attracting pollinators and commercial value. However, some genes involved in rose floral scent metabolism are less well understood. Here, wild-type scented rose (WR) and its spontaneous non-scented mutant rose (MR) were analyzed. SPME-GC/MS analysis showed that relative content of 1-ethenyl-4-methoxy-benzene represented was significantly different between WR and MR. We have isolated an EST encoding a MYB family of transcription factor from SSH libraries of the two roses in the previous studies, and designated RhMYB1. In the study, the full-length cDNA of RhMYB1 was identified and characterized by rapid amplification of cDNA ends (RACE). The RhMYB1 full-length cDNA was 1,125 bp containing an 882 bp open reading frame, which encodes a precursor protein of 294 amino acids. Sequence alignments revealed that RhMYB1 shared high similarity with other plants R-type MYB, and RhMYB1 contained a DNA binding domain. Northern blot analysis revealed that RhMYB1 was expressed specifically in flower petal, moreover, the expression level of RhMYB1 in WR increased along with scent emission, and decreased when the scent emission decreased. It is suggested that RhMYB1 might be a putative identification of gene involved in the biosynthesis of rose scent.
Similar content being viewed by others
References
Weiss EA (1997) Rosaceae. In: Weiss EA (ed) Essential oil crops. CAB International Wallingford, Oxon, UK, pp 393–416
Gudin S (2000) Rose: genetics and breeding. Plant Breed Rev 17:159–189
Bergougnoux V, Caissard JC, Jullien F, Magnard JL, Scalliet G, Cock JM, Hugueney P, Baudino S (2007) Both the adaxial and abaxial epidermal layers of the rose petal emit volatile scent compounds. Planta 226:853–866
Guterman I, Shalit M, Menda N, Piestun D, Dafny-Yelin M, Shalev G, Bar E, Davydov O, Ovadis M, Emanuel M, Wang J, Adam Z, Pichersky E, Lewinsohn E, Zamir D, Vainstein A, Weiss D (2002) Rose scent: genomics approach to discovering novel floral fragrance-related genes. Plant Cell 14:2325–2338
Channelière S, Rivière S, Scalliet G, Szecsi J, Jullien F, Dolle C, Vergne P, Dumas C, Bendahmane M, Hugueney P, Cock JM (2002) Analysis of gene expression in rose petals using expressed sequence tags. FEBS Lett 515:35–38
Flament I, Debonneville C, Furrer A (1993) Volatile constituents of roses: characterization of cultivars based on the headspace analysis of living flower emissions. In: Teranishi R, Buttery RG, Sugisawa H (eds) Bioactive volatile compounds from plants. American Chemical Society, Washington, DC, pp 269–281
Scalliet G, Journot N, Jullien F, Baudino S, Magnard JL, Channelière S, Vergne P, Dumas C, Bendahmane M, Cock JM, Hugueney P (2002) Biosynthesis of the major scent components 3,5-dimethoxytoluene and 1,3,5-trimethoxybenzene by novel rose O-methyltransferases. FEBS Lett 523:113–118
Baudino S, Caissard JC, Bergougnoux V, Jullien F, Magnard JL, Scalliet G, Cock JM, Hugueney P (2007) Production and emission of volatile compounds by petal cells. Plant Signal Behav 2:525–526
Brunke EJ, Hammerschmidt FJ, Schmaus G (1992) Scent of roses recent results. Flavour Frag J 7:195–198
Picone JM, Clery RA, Watanabe N, MacTavish HS, Turnbull CG (2004) Rhythmic emission of floral volatiles from Rosa damascena semperflorens cv. ‘Quatre Saisons’. Planta 219:468–478
Dudareva N, Cseke L, Blanc VM, Pichersky E (1996) Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell 8:1137–1148
Pichersky E, Noel JP, Dudareva N (2006) Biosynthesis of plant volatiles: nature’s diversity and ingenuity. Science 311:808–811
Vainstein A, Lewinsohn E, Weiss D (2006) An integrated genomics approach to identifying floral scent genes in rose. In: Dudareva N, Pichersky E (eds) Biology of floral scent. CRC Press–Taylor & Francis Group, Boca Raton, pp 91–102
Scalliet G, Piola F, Douady CJ, Réty S, Raymond O, Baudino S, Bordji K, Bendahmane M, Dumas C, Cock JM, Hugueney P (2008) Scent evolution in Chinese roses. Proc Natl Acad Sci USA 105:5927–5932
Scalliet G, Lionnet C, Le Bechec M, Dutron L, Magnard JL, Baudino S, Bergougnoux V, Jullien F, Chambrier P, Vergne P, Dumas C, Cock JM, Hugueney P (2006) Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent. Plant Physiol 140:18–29
Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Adam Z, Zamir D, Vainstein A, Weiss D, Pichersky E, Lewinsohn E (2003) Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/Citronellol acetyltransferase in developing rose petals. Plant Physiol 131:1868–1876
Farhi M, Lavie O, Masci T, Hendel-Rahmanim K, Weiss D, Abeliovich H, Vainstein A (2010) Identification of rose phenylacetaldehyde synthase by functional complementation in yeast. Plant Mol Biol 72:235–245
Wei YL, Li JN, Lu J, Tang ZL, Pu DC, Chai YR (2007) Molecular cloning of Brassica napus TRANSPARENT TESTA 2 gene family encoding potential MYB regulatory proteins of proanthocyanidin biosynthesis. Mol Biol Rep 34:105–120
Mehrtens F, Kranz H, Bednarek P, Weisshaar B (2005) The Arabidopsis transcription factor MYB12 is a flavonil-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138:1083–1096
Verdonk JC, Haring MA, van Tunen AJ, Schuurink RC (2005) ODORANT1 regulates fragrance biosynthesis in petunia flowers. Plant Cell 17:1612–1624
Xie JR, Liang GL, Li SF, Cheng ZQ, Tang KX, Huang XQ (2007) Identification of pink sprout mutation from ‘Gold Island’ Rose. North Hortic 11:186–188
Wady L, Bunte A, Pehrson C, Larsson L (2003) Use of gas chromatography-mass spectrometry/solid phase micro-extraction for the identification of MVOCs from moldy building materials. J Microbiol Methods 52:325–332
Zhu B, Xiong AS, Peng RH, Xu J, Jin XF, Meng XR, Yao QH (2010) Over-expression of ThpI from Choristoneura fumiferana enhances tolerance to cold in Arabidopsis. Mol Biol Rep 37(2):961–966
Xie JR, Xiong YH, Liang GL, Cheng ZQ, Tang KX, Huang XQ (2008) Identification of differentially expressed genes in fragrant rose Jinyindao with suppressive subtraction hybridization. Sci Hortic l16:318–323
Rabinowicz PD, Braun EL, Wolfe AD, Bowen B, Grotewold E (1999) Maize R2R3 Myb genes: sequence analysis reveals amplification in the higher plants. Genetics 153:427–444
Yi J, Derynck MR, Li X, Telmer P, Marsolais F, Dhaubhadel S (2010) A single-repeat MYB transcription factor, GmMYB176, regulates CHS8 gene expression and affects isoflavonoid biosynthesis in soybean. Plant J 62:1019–1034
van Schie CC, Haring MA, Schuurink RC (2006) Regulation of terpenoid and benzenoid production in flowers. Curr Opin Plant Biol 9:203–208
Yokoya K, Roberts AV, Mottley J, Lewis R, Brandham PE (2000) Nuclear DNA amounts in roses. Ann Bot 85:557–561
Ben Zvi MM, Negre-Zakharov F, Masci T, Ovadis M, Shklarman E, Ben-Meir H, Tzfira T, Dudareva N, Vainstein A (2008) Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowers. Plant Biotechnol J 6:403–415
Davuluri GR, van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Brummell DA, King SR, Palys J, Uhlig J, Bramley PM, Pennings HM, Bowler C (2005) Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol 23:890–895
Hoballah ME, Gübitz T, Stuurman J, Broger L, Barone M, Mandel T, Dell’Olivo A, Arnold M, Kuhlemeier C (2007) Single gene-mediated shift in pollinator attraction in Petunia. Plant Cell 19:779–790
Wan L, Xia X, Hong D, Yang G (2010) Molecular analysis and expression of a floral organ-specific polygalacturonase gene isolated from rapeseed (Brassica napus L). Mol Biol Rep 37(8):3851–3862. doi:10.1007/s11033-010-0041-2
Zuker A, Tzfira T, Ben-Meir H, Ovadis M, Shklarman E, Itzhaki H, Forkmann G, Martens S, Neta-Sharir I, Weiss D, Vainstein A (2002) Modification of flower color and fragrance by antisense suppression of the flavanone 3-hydroxylase gene. Mol Breeding 9:33–41
Dudareva N, Pichersky E, Gershenzon J (2004) Biochemistry of plant volatiles. Plant Physiol 135:1893–1920
Flament I, Debonneville C, Furrer A (1993) Volatile constituents of roses: characterization of cultivars based on the headspace analysis of living flower emissions. In: Teranishi R, Buttery RG, Sugisawa H (eds) Bioactive volatile compounds from plants. American Chemical Society, Washington, DC, pp 269–281
Blount JW, Korth KL, Masoud SA, Rasmussen S, Lamb C, Dixon RA (2000) Altering expression of cinnamic acid 4-hydroxylase in transgenic plants provides evidence for a feedback loop at the entry point into the phenylpropanoid pathway. Plant Physiol 122:107–116
Schuurink RC, Haring MA, Clark DG (2006) Regulation of volatile benzenoid biosynthesis in petunia flowers. Trends Plant Sci 11:20–25
Hendel-Rahmanim K, Masci T, Vainstein A, Weiss D (2007) Diurnal regulation of scent emission in rose flowers. Planta 226:1491–1499
Lavid N, Wang J, Shalit M, Guterman I, Bar E, Beuerle T, Menda N, Shafir S, Zamir D, Adam Z, Vainstein A, Weiss D, Pichersky E, Lewinsohn E (2002) O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals. Plant Physiol 129:1899–1907
Acknowledgments
We thank Dr. Guo-feng Liu, Huazhong Agricultural University (People’s Republic of China) for proofreading of the manuscript, and Dr. Ji-rong Xie, Chongqing University of Arts and Sciences (People’s Republic of China) for advising the work. This study was supported by ministry of agriculture project (200903020), Provincial Natural Science Foundation of Yunnan Province, People’s Republic of China (2007C0003Z) and the Natural Science Foundation of China (31060268).
Author information
Authors and Affiliations
Corresponding author
Additional information
The authors Huijun Yan and Hao Zhang contributed equally to this work.
Rights and permissions
About this article
Cite this article
Yan, H., Zhang, H., Wang, Q. et al. Isolation and identification of a putative scent-related gene RhMYB1 from rose. Mol Biol Rep 38, 4475–4482 (2011). https://doi.org/10.1007/s11033-010-0577-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-010-0577-1