Skip to main content
SpringerLink
Log in

Rhythmic emission of floral volatiles from Rosa damascena semperflorens cv. ‘Quatre Saisons’

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

The control of rhythmic emission of floral volatiles emitted from Rosa damascena semperflorens cv. ‘Quatre Saisons’ throughout floral development under various light regimes was studied. 2-Phenylethanol was the major volatile emitted in addition to monoterpenols, oxidised monoterpenols, monoterpenes and aromatic compounds. All detected volatiles were emitted rhythmically, with maximum peaks coinciding 8–10 h into a 12-h photoperiod. For some compounds a secondary, nocturnal peak was apparent. The primary and secondary maxima both occurred at approximately 24-h intervals. Rhythms appeared to be regulated endogenously: rhythmic emission continued upon exposure to continuous light or continuous darkness, and a phase shift in emission was induced upon inversion of the photoperiod. Additionally, emission continued after flower excision. A similar profile of free volatiles was stored within the floral tissue, together with glycosidic forms of 2-phenylethanol (>99% β-d-glucoside), benzyl alcohol, citronellol and geraniol. Regression analysis indicated a significant decrease in glycosylated 2-phenylethanol through the photoperiod. These results suggest that glycosylated volatiles stored within petals may be a source of rhythmically emitted volatiles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2a–d
Fig. 3a–d
Fig. 4a–c
Fig. 5a–c

Similar content being viewed by others

Abbreviations

CD :

Continuous darkness

CL :

Continuous light

2-PE :

2-Phenylethanol

2-PEG :

2-Phenylethyl β-d-glucopyranoside

References

  • Ackermann IE, Banthorpe DV, Fordham WD, Kinder JP, Poots I (1989) Beta-glucosides of aroma components from petals of Rosa species—assay, occurrence, and biosynthetic implications. J Plant Physiol 134:567–572

    CAS  Google Scholar 

  • Altenburger R, Matile P (1988) Circadian rhythmicity of fragrance emission in flowers of Hoya carnosa R. Br. Planta 174:248-252

    CAS  Google Scholar 

  • Borgkarlson AK, Valterova I, Nilsson LA (1994) Volatile compounds from flowers of 6 species in the family Apiaceae—bouquets for different pollinators. Phytochemistry 35:111–119

    Article  CAS  Google Scholar 

  • Dudareva N, Murfitt LM, Mann CJ, Gorenstein N, Kolosova N, Kish CM, Bonham C, Wood K (2000) Developmental regulation in methyl benzoate biosynthesis and emission in snapdragon flowers. Plant Cell 12:949–961

    Article  CAS  PubMed  Google Scholar 

  • Ervik F, Tollsten L, Knudsen JT (1999) Floral scent chemistry and pollination ecology in phytelephantoid palms (Arecaceae). Plant Syst Evol 217:279–297

    Google Scholar 

  • Francis MJO, Allcock C (1969) Geranyl-β-d-glucoside: occurrence and synthesis in rose flowers. Phytochemistry 8:1339–1347

    Article  CAS  Google Scholar 

  • Galen C (1996) Rates of floral evolution: adaptation to bumblebee pollination in an alpine wildflower, Polemonium viscosum. Evolution 50:120–125

    Google Scholar 

  • Hansted L, Jakobsen HB, Olsen CE (1994) Influence of temperature on the rhythmic emission of volatiles from Ribes nigrum flowers in situ. Plant Cell Environ 17:1069–1072

    CAS  Google Scholar 

  • Helsper JPFG, Davies JA, Bouwmeester HJ, Krol AF, van Kampen MH (1998) Circadian rhythmicity in emission of volatile compounds by flowers of Rosa hybrida L. cv. Honesty. Planta 207:88–95

    Article  CAS  Google Scholar 

  • Helsper JPFG, Davies JA, Verstappen FWA (2002) Analysis of rhythmic emission of volatile compounds of rose flowers. In: Jackson JF, Linskens HF (eds) Molecular methods in plant analysis, vol 21. Springer, Berlin Heidelberg New York, pp 199–221

  • Jakobsen H, Christensen LF (2002) Diurnal changes in the concentrations of 2-phenylethyl β-d-glucopyranoside and the corresponding volatile aglycone in the tissue and headspace of Trifolium repens L. florets. Plant Cell Environ 25:773–781

    Article  CAS  Google Scholar 

  • Jakobsen H, Olsen C (1994) Influence of climatic factors on emission of flower volatiles in situ. Planta 192:365–371

    CAS  Google Scholar 

  • Jones MB, Mansfield TA (1975) Circadian rhythms in plants. Sci Prog Oxford 62:103–125

    CAS  Google Scholar 

  • Jurgens A, Webber AC, Gottsberger G (2000) Floral scent compounds of Amazonian Annonaceae species pollinated by small beetles and thrips. Phytochemistry 55:551–558

    Article  CAS  PubMed  Google Scholar 

  • Knudsen JT, Tollsten L, Bergstrom LG (1993) Floral scents—a checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33:253–280

    CAS  Google Scholar 

  • Kolosova N, Gorenstein N, Kish CM, Dudareva N (2001) Regulation of circadian methyl benzoate emission in diurnally and nocturnally emitting plants. Plant Cell 13:2333–2347

    Article  CAS  PubMed  Google Scholar 

  • Loughrin JH, Hamilton-Kemp TR, Andersen RA, Hildebrand DF (1991) Circadian rhythm of volatile emission from flowers of Nicotiana sylvestris and N. suaveolens. Physiol Plant 83:492–496

    Article  CAS  Google Scholar 

  • Loughrin JH, Hamilton-Kemp TR, Burton HR, Andersen RA, Hildebrand DF (1992) Glycosidically bound volatile components of Nicotiana sylvestris and N. suaveolens flowers. Phytochemistry 31:1537–1540

    Article  CAS  Google Scholar 

  • MacTavish HS, Davies NW, Menary RC (2000) Emission of volatiles from brown boronia flowers: some comparative observations. Ann Bot 86:347–354

    Article  Google Scholar 

  • Matile P, Altenburger R (1988) Rhythms of fragrance emission in flowers. Planta 174:242–247

    CAS  Google Scholar 

  • Miyake T, Yamaoka R, Yahara T (1998) Floral scents of hawkmoth-pollinated flowers in Japan. J Plant Res 111:199–205

    CAS  Google Scholar 

  • Odell E, Raguso RA, Jones KN (1999) Bumblebee foraging responses to variation in floral scent and color in snapdragons (Antirrhinum: Scrophulariaceae). Am Midl Nat 142:257–265

    Google Scholar 

  • Oka N, Ohishi H, Hatano T, Hornberger M, Sakata K, Watanabe N (1999) Aroma evolution during flower opening in Rosa damascena Mill. Z Naturforsch Teil C 54:1–7

    Google Scholar 

  • Omura H, Honda K, Hayashi N (2000) Floral scent of Osmanthus fragrans discourages foraging behavior of cabbage butterfly, Pieris rapae. J Chem Ecol 26:655–666

    Article  CAS  Google Scholar 

  • Overland L (1960) Endogenous rhythm in opening and odor of flowers of Cestrum nocturnum. Am J Bot 47:378–382

    Google Scholar 

  • Reuveni M, Sagi Z, Evnor D, Hetzroni A (1999) β-glucosidase activity is involved in scent production in Narcissus flowers. Plant Sci 147:19–24

    Article  CAS  Google Scholar 

  • Schiestl F, Ayasse M, Paulus H, Erdmann D, Francke W (1997) Variation of floral scent emission and postpollination changes in individual flowers of Ophrys sphegodes subsp. sphegodes. J Chem Ecol 23:2881–2895

    CAS  Google Scholar 

  • Watanabe N, Watanabe S, Nakajima R, Moon JH, Shimokihara K, Inagaki J, Etoh H, Asai T, Sakata K, Ina K (1993) Formation of flower fragrance compounds from their precursors by enzymatic action during flower opening. Biosci Biotechnol Biochem 57:1101–1106

    CAS  Google Scholar 

  • Watanabe S, Hashimoto I, Hayashi K, Yagi K, Asai T, Knapp H, Straubinger M, Winterhalter P, Watanabe N (2001) Isolation and identification of 2-phenylethyl disaccharide glycosides and mono glycosides from rose flowers, and their potential role in scent formation. Biosci Biotechnol Biochem 65:442–445

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agricultural Sciences, Imperial College London, Wye Campus, Wye, Kent, TN25 5AH, UK

    Joanne M. Picone & Colin G. N. Turnbull

  2. Quest International, Ashford, Kent, TN24 0LT, UK

    Robin A. Clery

  3. Department of Applied Biological Chemistry, Shizuoka University, 422-8529, Shizuoka, Japan

    Naoharu Watanabe

  4. ADAS Consulting Ltd., ADAS Arthur Rickwood, Mepal, Ely, Cambridgeshire, CB6 2BA, UK

    Hazel S. MacTavish

Authors
  1. Joanne M. Picone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robin A. Clery
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Naoharu Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hazel S. MacTavish
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Colin G. N. Turnbull
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Colin G. N. Turnbull.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Picone, J.M., Clery, R.A., Watanabe, N. et al. Rhythmic emission of floral volatiles from Rosa damascena semperflorens cv. ‘Quatre Saisons’. Planta 219, 468–478 (2004). https://doi.org/10.1007/s00425-004-1250-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00425-004-1250-5

Keywords

Search

Navigation