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Primary molecular features of self-incompatible and self-compatible F1 seedling from apricot (Prunus armeniaca L.) Katy × Xinshiji

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Abstract

Expression of the S-RNase genes in the self-compatible (SC) apricot (Prunus armeniaca L.) cultivar Katy, the self-incompatible (SI) cultivar Xinshiji and their F1 seedling was examined in this study. Three S-genotypes, S9Sc (Sc, self-compatibility S-gene absent from the style), S8S9, and S8S10, were obtained. Seedlings with S-RNase that migrated as a single band in gel electrophoresis were SC, despite high transcript abundance, and those with S-RNase that migrated as two bands were SI with high transcript abundance or SC with low transcript expression. S8-RNase was induced in SI cultivars only 24 h after self-pollination, indicating post-transcriptional regulation of S8-RNase in SI apricots. A Proteomic study showed that 35 protein spots were synthesized differently between SC and SI pistils. Fifteen of the 35 protein spots were identified; nine proteins, including receptor protein kinase-like protein, reversibly glycosylated polypeptide-2, and isoflavone reductase-like protein, were detected only in the SC pistils; while nine proteins, including actin 7, a putative serine/threonine kinase, and S-RNase, were detected only in the SI pistils. A mitochondrial NAD-dependent malate dehydrogenase and a probable elongation factor G were up-regulated, while heat shock cognate 70 was down-regulated in the SC pistils compared to those in the SI pistils. The results suggest that the proteins responsible for self-compatibility and self-incompatibility may be different.

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Abbreviations

ACN:

Acetonitrile

BB:

Bromophenol blue

BS:

At balloon stage

DTT:

Dithiothreitol

FQRT-PCR:

Fluorescence quantification RT-PCR technology

HPLC:

High-pressure liquid chromatography

IAM:

Iodoacetamide

IEF:

Isoelectric focusing electrophoresis

LC-ESI-MS/MS:

Liquid chromatography-electrospray ion trap tandem mass spectrometry

MS:

Mass spectrometer

PMSF:

Phenylmethyl sulfonyl fluoride

RT-PCR:

Reverse transcript-PCR

SI:

Self-incompatible

SC:

Self-compatible

TFA:

Triflouroacetic acid

2-DE:

Two-dimensional gel electrophoresis

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Acknowledgements

The research was supported by the National Natural Science Foundation (No. 30370992 and 30760143), the Special Program for Doctorial Site of Universities (No. 200404344011) and the Key Project for Excellent College Teachers from Ministry of Education, China (No. 204178), which are gratefully acknowledged. The authors sincerely thank Dr. Guanggan Hu in Michael Smith Laboratories, the University of British Columbia, Canada for his carefully reading of this manuscript.

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Authors and Affiliations

  1. Horticultural Department, Shihezi University, Shihezi, Xinjiang, 832003, China

    J. R. Feng

  2. Horticultural Science and Engineering College, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, China

    X. S. Chen, Z. H. Yuan, L. J. Zhang, Z. J. Ci, X. L. Liu & C. Y. Zhang

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  1. J. R. Feng
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Correspondence to X. S. Chen.

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Feng, J.R., Chen, X.S., Yuan, Z.H. et al. Primary molecular features of self-incompatible and self-compatible F1 seedling from apricot (Prunus armeniaca L.) Katy × Xinshiji. Mol Biol Rep 36, 263–272 (2009). https://doi.org/10.1007/s11033-007-9175-2

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  • DOI: https://doi.org/10.1007/s11033-007-9175-2

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