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Identification and validation of breeder-friendly DNA markers for Pl arg gene in sunflower

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Abstract

Downy mildew is a fungal disease of sunflower that can lead to severe yield losses. The damage caused by the pathogen can be controlled by growing resistant sunflower varieties. Gene Pl arg was introgressed into cultivated sunflower from the wild species Helianthus argophyllus and provides resistance against all known downy mildew races. In this study, we used a mapping population from the cross-RHA 419/RHA-N-49. We identified a new co-segregating simple sequence repeat marker ORS675 and confirmed the co-segregation of markers ORS716 and ORS662 with Pl arg gene. The markers were validated on two registered resistant inbred lines RHA 443 and RHA 464, as well as on twenty inbred lines RH 1–20 obtained through methods of classical breeding. Molecular marker ORS716 was assessed for usefulness in selecting resistant progeny in 12 BC populations. Markers were found to be valuable for molecular breeding in diverse genetic backgrounds and enabled transfer of the resistance gene in different sunflower genotypes.

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Acknowledgments

The authors are grateful to Mr. Zvonimir Sakac for technical assistance in preparation of the manuscript. This work was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, project TR 31025.

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Authors and Affiliations

  1. Institute of Field and Vegetable Crops, 21000, Novi Sad, Serbia

    Ivana Imerovski, Aleksandra Dimitrijevic, Dragana Miladinovic, Sinisa Jocic, Bosko Dedic & Sandra Cvejic

  2. Faculty of Agriculture, University of Belgrade, 11080, Zemun, Serbia

    Gordana Surlan-Momirovic

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  1. Ivana Imerovski
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  2. Aleksandra Dimitrijevic
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  3. Dragana Miladinovic
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  4. Sinisa Jocic
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  6. Sandra Cvejic
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  7. Gordana Surlan-Momirovic
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Correspondence to Dragana Miladinovic.

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Imerovski, I., Dimitrijevic, A., Miladinovic, D. et al. Identification and validation of breeder-friendly DNA markers for Pl arg gene in sunflower. Mol Breeding 34, 779–788 (2014). https://doi.org/10.1007/s11032-014-0074-7

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