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The genetic architecture of seedling resistance to Septoria tritici blotch in the winter wheat doubled-haploid population Solitär × Mazurka

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Abstract

Breeding for resistance to Septoria tritici blotch (STB), caused by Mycosphaerella graminicola (anamorph: Septoria tritici), is an essential component in controlling this important foliar disease of wheat. Inheritance of seedling resistance to seven worldwide pathogen isolates has been studied in a doubled-haploid (DH) population derived from a cross between the field resistant cultivar Solitär and the susceptible cultivar Mazurka. Multiple quantitative trait locus (QTL) mapping revealed major and minor genetic effects on resistance as well as several epistatic relationships in the seedling stage. Solitär conferred resistance to isolate IPO323, governed by Stb6 on chromosome 3A, as well as to IPO99015, IPO92034, Hu1 and Hu2 controlled by a QTL on chromosome arm 1BS, possibly corresponding to Stb11 and minor QTL on chromosomes 1B, 3D, 6B and 7D. Resistance of Mazurka to IPO90015 and BBA22 was caused by a QTL located in a region on 4AL which harbours Stb7 or Stb12. QTL specific to pycnidial coverage on 3B and specific to necrosis on 1A could be discovered for isolate IPO92034. Pairwise epistatic interactions were reliably detected with five isolates. Although their contributions to the total variance are generally low, the genotypic effect of the QTL by QTL interaction of 4AL (Stb7 or Stb12) and 3AS (Stb6) made up almost 15% of disease expression. Altogether, the results suggest a complex inheritance of resistance to STB in the seedling stage in terms of isolate-specificity and resistance mechanisms, which have implications for marker-assisted breeding in an attempt to pyramid STB resistance genes.

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Acknowledgments

We thank Hubert Kempf (Secobra Saatzucht, Feldkirchen) and Laszlo Láng (ARI Martonvásár) for providing seed of Solitär and Mazurka, and express thanks to John Speakman (BASF Ludwigshafen) and Bernd Rodemann (JKI Braunschweig) for providing fungal isolates. We are also indebted to Carsten Springmann and Viktor Korzun (KWS-Planta, KWS-Lochow) for successful establishment of the DH lines, and Genoplante-Valor (Evry, France) for supplying Xgpw microsatellite primers. The technical assistance of Sebastian Kosellek, Helga Sängerlaub, Brigitte Schröder and Bernd Kollmorgen is greatly acknowledged. We are also grateful to staff members of PRI Wageningen and of IPK Gatersleben. We say thanks to Tim March for critical reading of the manuscript. Funding of this research, conducted in the PlantResourceII framework, was supported by the Land Sachsen-Anhalt, project number FAZ: 3593B/0405T, and a DAAD fellowship to CK. S Mahmod Tabib G. is funded by GIE Bioplante and Fonds de Soutien à l’Obtention Végétale (FSOV), France.

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  1. Department of Plant Breeding, Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Betty-Heimann-Str. 3, 06120, Halle (Saale), Germany

    Christiane Kelm, Sebastian Miersch & W. Eberhard Weber

  2. Plant Science Group, Plant Research International BV, Wageningen University, Droevendaalsesteeg 1, 06708 PB, Wageningen, The Netherlands

    S. Mahmod Tabib Ghaffary & Gert H. J. Kema

  3. Institute of Biology/Geobotany and Botanical Garden, Martin-Luther-University Halle-Wittenberg, Am Kirchtor 1, 06108, Halle (Saale), Germany

    Helge Bruelheide

  4. Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany

    Marion S. Röder

  5. Saatzucht Steinach GmbH & Co KG, Wittelsbacherstr. 15, 94377, Steinach, Germany

    Bernhard Saal

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  1. Christiane Kelm
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  2. S. Mahmod Tabib Ghaffary
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Kelm, C., Ghaffary, S.M.T., Bruelheide, H. et al. The genetic architecture of seedling resistance to Septoria tritici blotch in the winter wheat doubled-haploid population Solitär × Mazurka. Mol Breeding 29, 813–830 (2012). https://doi.org/10.1007/s11032-011-9592-8

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