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Chromosomal location of a race-specific resistance gene to Mycosphaerella graminicola in the spring wheat ST6

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Abstract

Septoria tritici blotch, caused by Mycosphaerella graminicola, is a serious foliar disease of wheat worldwide. Qualitative, race-specific resistance sources have been identified and utilized for resistant cultivar development. However, septoria tritici blotch resistant varieties have succumbed to changes in virulence of M. graminicola on at least three continents. The use of resistance gene pyramids may slow or prevent the breakdown of resistance. A clear understanding of the genetics of resistance and the identification of linked PCR-based markers will facilitate the recovery of wheat lines carrying multiple septoria tritici blotch resistance genes. The resistance gene in ST6 to isolate MG2 of M. graminicola was mapped with microsatellite markers in two populations, ST6/Erik and ST6/Katepwa. Bulk segregant analysis identified a marker on chromosome 4AL putatively linked to the resistance gene. A large linkage group was identified in each population using additional microsatellite markers mapping to chromosome 4AL. The resistance gene in ST6 mapped to the distal end of chromosome 4AL in each mapping population and was designated Stb7. Three of the microsatellite loci, Xwmc313, Xwmc219 and Xgwm160, mapped within 3.5 cM of Stb7; however, none flanked Stb7. Xwmc313 was the closest and mapped 0.3 and 0.5 cM from Stb7 in the crosses ST6/Katepwa and ST6/Erik, respectively. WMC313 will be very useful for marker-assisted selection of Stb7 in Canadian breeding programs because the ST6 allele of Xwmc313 was not identified in any of the Canadian common wheat cultivars tested.

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Acknowledgements

We thank Sejet Plant Breeding (Denmark) for permission to publish the primer sequences of WMC313. Thanks also to the Natural Sciences and Engineering Research Council of Canada, the Western Grains Research Foundation, Agriculture and Agri-Food Canada and the Canadian Wheat Board for their financial support.

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Authors and Affiliations

  1. Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada

    C. A. McCartney, A. L. Brûlé-Babel & L. Lamari

  2. Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Road, Winnipeg, MB R3T 2M9, Canada

    D. J. Somers

Authors
  1. C. A. McCartney
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  2. A. L. Brûlé-Babel
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  3. L. Lamari
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  4. D. J. Somers
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Correspondence to A. L. Brûlé-Babel.

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Communicated by P. Langridge

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McCartney, C.A., Brûlé-Babel, A.L., Lamari, L. et al. Chromosomal location of a race-specific resistance gene to Mycosphaerella graminicola in the spring wheat ST6. Theor Appl Genet 107, 1181–1186 (2003). https://doi.org/10.1007/s00122-003-1359-0

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  • DOI: https://doi.org/10.1007/s00122-003-1359-0

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