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Molecular markers for wheat leaf rust resistance gene Lr41

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Abstract

Leaf rust, caused by Puccinia triticina Eriks., is an important foliar disease of common wheat (Triticum aestivum L.) worldwide. Pyramiding several major rust-resistance genes into one adapted cultivar is one strategy for obtaining more durable resistance. Molecular markers linked to these genes are essential tools for gene pyramiding. The rust-resistance gene Lr41 from T. tauschii has been introgressed into chromosome 2D of several wheat cultivars that are currently under commercial production. To discover molecular markers closely linked to Lr41, a set of near-isogenic lines (NILs) of the hard winter wheat cultivar Century were developed through backcrossing. A population of 95 BC3F2:6 NILs were evaluated for leaf rust resistance at both seedling and adult plant stages and analyzed with simple sequence repeat (SSR) markers using bulked segregant analysis. Four markers closely linked to Lr41 were identified on chromosome 2DS; the closest marker, Xbarc124, was about 1 cM from Lr41. Physical mapping using Chinese Spring nullitetrasomic and ditelosomic genetic stocks confirmed that markers linked to Lr41 were on chromosome arm 2DS. Marker analysis in a diverse set of wheat germplasm indicated that primers BARC124, GWM210, and GDM35 amplified polymorphic bands between most resistant and susceptible accessions and can be used for marker-assisted selection in breeding programs.

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Acknowledgments

Nullitetrasomic and ditelosomic genetic stocks were provided by the Wheat Genetics and Genomics Resources Center of Kansas State University, Manhattan, KS. This project was partly funded by the National Research Initiative of USDA’s Cooperative State Research, Education and Extension Service, CAP grant number 2006-55606-16629. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No. 08-354-J from the Kansas Agricultural Experiment Station, Manhattan, KS, USA.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Xiaochun Sun & Guihua Bai

  2. USDA-ARS Plant Science and Entomology Research Unit, Manhattan, KS, 66506, USA

    Guihua Bai

  3. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Brett F. Carver

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  1. Xiaochun Sun
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  2. Guihua Bai
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  3. Brett F. Carver
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Correspondence to Guihua Bai.

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Sun, X., Bai, G. & Carver, B.F. Molecular markers for wheat leaf rust resistance gene Lr41 . Mol Breeding 23, 311–321 (2009). https://doi.org/10.1007/s11032-008-9237-8

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  • DOI: https://doi.org/10.1007/s11032-008-9237-8

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