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Molecular markers for adult plant leaf rust resistance gene Lr48 in wheat

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Abstract

Leaf rust of wheat, caused by Puccinia triticina, is an important disease throughout the world. The adult plant leaf rust resistance gene Lr48 reported in CSP44 was previously mapped in chromosome 2B, but the marker–gene association was weak. In this study, we confirmed the location of Lr48 to be in the short arm of chromosome 2B and identified closely linked markers suitable for use in breeding. The CSP44/WL711 recombinant inbred line (RIL) population (90 lines) showed monogenic segregation for Lr48. Twelve resistant and 12 susceptible RILs were used for selective genotyping using an iSelect 90K Infinium SNP assay. Closely linked SNPs were converted into Kompetitive allele-specific primers (KASP) and tested on the parental lines. KASP markers giving clear clusters for alternate genotypes were assayed on the entire RIL population. SNP markers IWB31002, IWB39832, IWB34324, IWB72894 and IWB36920 co-segregated with Lr48 and the marker IWB70147 was mapped 0.3 cM proximal to this gene. Closely linked KASP markers were tested on a set of Australian and Nordic wheat genotypes. The amplification of SNP alleles alternate to those linked with Lr48 in the majority of the Australian and Nordic wheat genotypes demonstrated the usefulness of these markers for marker-assisted pyramiding of Lr48 with other rust resistance genes.

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References

  • Bansal UK, Hayden MJ, Venkata BP, Khanna R, Saini RG, Bariana HS (2008) Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat. Theor Appl Genet 117:307–312

    Article  CAS  PubMed  Google Scholar 

  • Bansal UK, Kazi AG, Singh B, Hare RA, Bariana HS (2014a) Mapping of durable stripe rust resistance in a durum wheat cultivar Wollaroi. Mol Breeding 33:51–59

    Article  CAS  Google Scholar 

  • Bansal UK, Wicker T, Keller B, Hayden M, Bariana HS (2014b) Molecular mapping of an adult plant stem rust resistance gene Sr56 in winter wheat cultivar Arina. Theor Appl Genet 127:1441–1448

    Article  CAS  PubMed  Google Scholar 

  • Bariana HS, McIntosh RA (1995) Genetics of adult plant stripe rust resistance in four Australian wheats and the French cultivar ‘Hybride de Bersee’. Plant Breeding 114:485–491

    Article  Google Scholar 

  • Bariana HS, Brown GN, Bansal UK, Miah H, Standen GE, Lu M (2007) Breeding for triple rust resistance wheat cultivars for Australia using conventional and marker assisted selection technologies. Aust J Agric Res 58:576–587

    Article  Google Scholar 

  • Bolton MD, Kolmer JA, Garvin DF (2008) Wheat leaf rust caused by Puccinia triticina. Mol Plant Pathol 9:563–575

    Article  PubMed  Google Scholar 

  • Chapman JA, Mascher M, Buluç A, Barry K, Georganas E, Session A, Strnadova V, Jenkins J, Sehgal S, Oliker L, Schmutz J, Yelick KA, Scholz U, Waugh R, Poland JA, Muehlbauer GJ, Stein N, Rokhsar DS (2015) A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome. Genome Biol 16:26

    Article  PubMed  PubMed Central  Google Scholar 

  • Cockram J, Scuderi A, Barber T, Furuki E, Gardner KA, Gosman N, Kowalczyk R, Phan HP, Rose GA, Tan K-C, Oliver RP, Mackay IJ (2015) Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multi-parent advanced generation intercross population. G3 Genes Genomes Genet 5:2257–2266

    Google Scholar 

  • Dhariwal R, Gahlaut V, Govindraj BR, Singh D, Mathur S, Vyas S, Bandopadhyay R, Khurana JP, Tyagi AK, Prabhu KV, Mukhopadhyay K, Balyan HS, Gupta PK (2015) Stage-specific reprogramming of gene expression characterizes Lr48-mediated adult plant leaf rust resistance in wheat. Funct Integr Genomics 15:233–245

    Article  CAS  PubMed  Google Scholar 

  • Gowda M, Zhao Y, Würschum T, Longin CFH, Miedaner T, Ebmeyer E, Schachschneider R, Kazman E, Schacht J, Martinant J-P, Mette MF, Reif JC (2014) Relatedness severely impacts accuracy of marker-assisted selection for disease resistance in hybrid wheat. Heredity 112:552–561

    Article  CAS  PubMed  Google Scholar 

  • Huerta-Espino J, Singh RP, Germán SS, McCallum BD, Park RF, Chen WQ, Bhardwaj SC, Goyeau H (2011) Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 179:143–160

    Article  Google Scholar 

  • Khan M, Iqbal M (2011) Breeding for drought tolerance in wheat (Triticum aestivum L.): constraints and future prospects. Front Agric China 5:31–34

    Article  Google Scholar 

  • Kolmer JA (2005) Tracking wheat rust on a continental scale. Curr Opin Plant Biol 8:441–449

    Article  PubMed  Google Scholar 

  • Kolmer JA, Mert Z, Akan K, Demir L, Unsal R, Sermet C, Keser M, Akin B, Morgounov A (2013) Virulence of Puccinia triticina in Turkey and leaf rust resistance in Turkish wheat cultivars. Eur J Plant Pathol 135:703–716

    Article  CAS  Google Scholar 

  • Kosambi DD (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Lowe I, Cantu D, Dubcovsky J (2011) Durable resistance to the wheat rusts: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes. Euphytica 179:69–79

    Article  PubMed  PubMed Central  Google Scholar 

  • Mammadov J, Aggarwal R, Buyyarapu R, Kumpatla S (2012) SNP markers and their impact on plant breeding. Intl J Plant Genom 2012:728398

    Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932

    Article  CAS  PubMed  Google Scholar 

  • McIntosh RA (1998) Breeding wheat for resistance to biotic stresses. Euphytica 100:19–34

    Article  Google Scholar 

  • McIntosh RA, Welling CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO, Melbourne

    Book  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers W, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. In: Proceedings of the 12th International wheat genetics symposium, Yokohama, Japan

  • McIntosh RA, Dubcovsky J, Rogers JW, Morris C, Appels R, Xia CX (2014) Catalogue of gene symbols for wheat: 2013–2014 supplement. KOMUGI Integrated Wheat Science Database. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp. Accessed 15 Aug 2015

  • Morozova O, Marra MA (2008) Applications of next-generation sequencing technologies in functional genomics. Genomics 92:255–264

    Article  CAS  PubMed  Google Scholar 

  • Murray GM, Brennan PJ (2009) Estimating disease losses to the Australian wheat industry. Australas Plant Pathol 38:558–570

    Article  Google Scholar 

  • Ortiz R, Braun HJ, Crossa J, Crouch HJ, Davenport G, Dixon J, Dreisigacker S, Duveiller E, He Z, Huerta J, Joshi KA, Kishii M, Kosina P, Manes Y, Mezzalama M, Morgounov A, Murakami J, Nicol J, Ferrara OG, Ortiz-Monasterio IJ, Payne ST, Penña JR, Reynolds PM, Sayre DK, Sharma CR, Singh PR, Wang J, Warburton M, Wu H, Iwanaga M (2008) Wheat genetic resources enhancement by the International Maize and Wheat Improvement Center (CIMMYT). Genet Resour Crop Evol 55:1095–1140

    Article  Google Scholar 

  • Pretorius ZA, Wilcoxson RD, Long DL, Schafer JF (1984) Detecting wheat leaf rust resistance gene Lr13 in seedlings. Plant Dis 68:585–586

    Article  Google Scholar 

  • Priyamvada A, Saharan MS, Tiwari R (2011) Durable resistance in wheat. Int J Genet Mol Biol 3:108–114

    Google Scholar 

  • Randhawa M, Bansal U, Valárik M, Klocová B, Doležel J, Bariana H (2014) Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat. Theor Appl Genet 127:317–324

    Article  CAS  PubMed  Google Scholar 

  • Roelfs AP, Singh PR, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico, p 81

    Google Scholar 

  • Saini RG, Kaur M, Singh B, Sharma S, Nanda GS, Nayar SK, Gupta AK, Nagrajan S (2002) Lr48 and Lr49, novel hypersensitive adult plant leaf rust resistance genes in wheat (Triticum aestivum L.). Euphytica 124:365–370

    Article  CAS  Google Scholar 

  • Samsampour D, Zanjani BM, Pallavi JK, Singh A, Charpe A, Gupta SK, Prabhu KV (2010) Identification of molecular markers linked to adult plant leaf rust resistance gene Lr48 in wheat and detection of Lr48 in Thatcher near isogenic line with gene Lr25. Euphytica 174:337–342

    Article  CAS  Google Scholar 

  • Singh RP, Dubin HJ (1997) Sustainable control of wheat diseases in Mexico. In: Memorias Primer Simposio Internacional de Trigo, 7–9 April 1997. Cd. Obregon Sonora, Mexico, pp 93–103

  • Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung 35:133–139

    CAS  Google Scholar 

  • Singh A, Pallavi JK, Gupta P, Prabhu KV (2011) Identification of microsatellite markers linked to leaf rust adult plant resistance (APR) gene Lr48 in wheat. Plant Breed 130:31–34

    Article  CAS  Google Scholar 

  • Singh A, Knox RE, DePauw RM, Singh AK, Cuthbert RD, Campbell HL, Singh D, Bhavani S, Fetch T, Clarke F (2013) Identification and mapping in spring wheat of genetic factors controlling stem rust resistance and the study of their epistatic interactions across multiple environments. Theor Appl Genet 126:1951–1964

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tiwari VK, Heesacker A, Riera-Lizarazu O, Gunn H, Wang S, Wang Y, Gu YQ, Paux E, Koo D-H, Kumar A, Luo M-C, Lazo G, Zemetra R, Akhunov E, Friebe B, Poland J, Gill BS, Kianian S, Leonard JM (2016) A whole-genome, radiation hybrid mapping resource of hexaploid wheat. Plant J. doi:10.1111/tpj.13153

    PubMed  Google Scholar 

  • Trick M, Adamski NM, Mugford SG, Jiang CC, Febrer M et al (2012) Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat. BMC Plant Biol 12:14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  • Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing C, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

Vallence Nsabiyera thanks AusAID for the scholarship award and NARO for study leave. We thank the Australian Grains Research and Development Corporation (GRDC) for financial support through the Australian Cereal Rust Control Program for this study.

Author contributions

VN drafted the manuscript; VN, HB and UB developed segregating population; VN, HB and UB did rust phenotyping; VN and NQ did marker work; UB, KF and MH designed primers; UB, HB and MH edited the manuscript.

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Authors and Affiliations

  1. Faculty of Agriculture and Environment, The University of Sydney Plant Breeding Institute-Cobbitty, Private Bag 4011, Narellan, NSW, 2567, Australia

    Vallence Nsabiyera, Naeela Qureshi, Harbans S. Bariana & Urmil K. Bansal

  2. Department of Economic Development, Jobs, Transport and Resources, La Trobe University AgriBio, Bundoora, VIC, 3083, Australia

    Debbie Wong, Kerrie L. Forrest & Mathew J. Hayden

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  1. Vallence Nsabiyera
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  2. Naeela Qureshi
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  3. Harbans S. Bariana
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  6. Mathew J. Hayden
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  7. Urmil K. Bansal
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Correspondence to Urmil K. Bansal.

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Nsabiyera, V., Qureshi, N., Bariana, H.S. et al. Molecular markers for adult plant leaf rust resistance gene Lr48 in wheat. Mol Breeding 36, 65 (2016). https://doi.org/10.1007/s11032-016-0488-5

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