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Collagen type III content of the long head of the biceps tendon as an indicator of glenohumeral arthritis

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Abstract

Tendonosis is characterized by an increase in collagen type III relative to collagen type I, but the precise ratio of collagen type I to collagen type III in pathologic shoulder tendons has not been reported. A tendon continuously bathed in an OA synovial environment may exhibit histomorphologic differences as compared to those from shoulders with a preserved articular surface. The purpose of this study was to evaluate the biochemical and histological differences in the proximal portion of the long head of the biceps tendon (LHBT) from a non-arthritic versus an arthritic glenohumeral joint. Sixteen patients undergoing shoulder surgery were prospectively enrolled in the study. Group-1 consisted of patients with glenohumeral OA and Group-2 consisted of patients without OA. The LHBT was tenodesed and excised tendon was fixed, embedded, sectioned (5 µm), deparaffinized, and used for histology (H&E and Masson trichrome staining) and immunofluorescence analysis to determine levels of collagen III, MMP-1, MMP-9, TIMP-1, and TIMP-2. Compared to the patients without arthritis, group-1 exhibited significant inflammation with extracellular matrix disorganization and significantly higher (P < 0.05) collagen III, MMP-2, and MMP-9 levels. No considerable difference in collagen I was observed between the two groups. TIMP-1 and TIMP-2 were completely absent in both the groups. The level of collagen subtypes varied markedly between LHBT tendons from patients with and without OA. Increased collagen III in the LHBT is associated with glenohumeral arthritis. Measuring collagen type in the LHBT may serve as a useful metric in the diagnosis and treatment of musculoskeletal pathology.

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Abbreviations

LHBT:

Long head of the biceps tendon

RCI:

Rotator cuff injury

OA:

Osteoarthritis

ECM:

Extracellular matrix

HIER:

Heat-induced epitope retrieval

MMP:

Matrix metalloproteases

TIMP:

Tissue inhibitors of matrix metalloproteases

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Acknowledgements

This work was supported by Creighton University LB692 Grant to MF Dilisio from the State of Nebraska. The content of this research article is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Clinical & Translational Science, Creighton University School of Medicine, Omaha, NE, USA

    Finosh G. Thankam, Devendra K. Agrawal & Matthew F. Dilisio

  2. Department of Orthopedic Surgery, Creighton University School of Medicine, Omaha, NE, USA

    Daugherty K. Evan & Matthew F. Dilisio

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  1. Finosh G. Thankam
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  2. Daugherty K. Evan
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  3. Devendra K. Agrawal
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  4. Matthew F. Dilisio
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Correspondence to Matthew F. Dilisio.

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All authors have read the journal’s policy on disclosure of potential conflicts of interest and the authors have no other relevant affiliations or financial involvement with any organization or entity with financial interest or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. All authors declare that there is no conflict. No writing assistance was utilized in the production of this manuscript.

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Thankam, F.G., Evan, D.K., Agrawal, D.K. et al. Collagen type III content of the long head of the biceps tendon as an indicator of glenohumeral arthritis. Mol Cell Biochem 454, 25–31 (2019). https://doi.org/10.1007/s11010-018-3449-y

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  • DOI: https://doi.org/10.1007/s11010-018-3449-y

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