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Collagens, Proteoglycans, MMP-2, MMP-9 and TIMPs in Human Achilles Tendon Rupture

  • Symposium: Molecular and Clinical Developments in Tendinopathy
  • Published:
Clinical Orthopaedics and Related Research

Abstract

Tendon integrity depends on the extracellular matrix (ECM) metabolism which is regulated by proteolytic enzymes. However, it is unclear which enzymes play a role in tendon rupture. We studied the ECM of 19 ruptured human Achilles tendons, comparing the composition of specimens harvested close to the rupture with specimens harvested from an apparently healthy area in the same tendon. We compared gene expression of collagen Type I, decorin, and versican including enzymes involved in their metabolism as matrix metalloproteases (MMP-2 and -9) and tissue inhibitory of metalloproteinase (TIMP-1 and -2) using real-time PCR, zymography and FACE analysis. We found greater gene expression of proteoglycan core protein decorin and versican, collagen Type I, MMPs and TIMPs in the tendon rupture. Zymography analysis, reflecting expression of enzymatic activity, confirmed the gene expression data at protein level. Carbohydrate content was greater in the macroscopically healthy area than in the ruptured area. In the ruptured area, we found increased core protein synthesis but without the normal glycosaminoglycan production. The tissue in the area of rupture undergoes marked rearrangement at molecular levels and supports the role of MMPs in the pathology.

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Authors and Affiliations

  1. Department of Experimental Biomedical and Clinical Sciences (DSBSC), University of Insubria, Varese, Italy

    Evgenia Karousou PhD, Davide Vigetti PhD & Alberto Passi MD, PhD

  2. Department of Orthopaedics and Traumatology, University of Insubria, Varese, Italy

    Mario Ronga MD

  3. Department of Trauma and Orthopaedic Surgery, Keele University School of Medicine, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke on Trent, ST4 7QB, Staffs, UK

    Nicola Maffulli MD, MS, PhD, FRCS(Orth)

Authors
  1. Evgenia Karousou PhD
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  2. Mario Ronga MD
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  3. Davide Vigetti PhD
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  4. Alberto Passi MD, PhD
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  5. Nicola Maffulli MD, MS, PhD, FRCS(Orth)
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Corresponding author

Correspondence to Nicola Maffulli MD, MS, PhD, FRCS(Orth).

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Each author certifies that he or she has no commercial associations (eg, consultancies, stock ownership, equity interest, patent/licensing arrangements, etc) that might pose a conflict of interest in connection with the submitted article.

Each author certifies that his or her institution has approved the reporting of these cases, that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participation in the study was obtained.

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Karousou, E., Ronga, M., Vigetti, D. et al. Collagens, Proteoglycans, MMP-2, MMP-9 and TIMPs in Human Achilles Tendon Rupture. Clin Orthop Relat Res 466, 1577–1582 (2008). https://doi.org/10.1007/s11999-008-0255-y

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  • DOI: https://doi.org/10.1007/s11999-008-0255-y

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