Abstract
Differential scanning calorimetry (DSC) provides a unique temperature-induced denaturation profile of the mixture of component proteins in the serum. This profile depends on a health status of serum donor, physicochemical properties of serum solution samples, and many experimental factors. Whether an exposure of serum sample to neutron radiation leads to changes in serum DSC profile has been checked in this study. The results indicate that immediately after the experiment, the differences between DSC serum profiles for irradiated and control samples are not significant. However, the distinct differences in the thermal unfolding of proteins present in the irradiated and control serum have been observed after 2 or 3 weeks of the storage of serum samples at about 4 °C. These differences have been found more pronounced at the dose of 5 Gy than at 0.5 Gy.
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Michnik, A., Polaczek-Grelik, K., Staś, M. et al. Delayed effects of neutron radiation on human serum. J Therm Anal Calorim 126, 37–45 (2016). https://doi.org/10.1007/s10973-016-5255-7
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DOI: https://doi.org/10.1007/s10973-016-5255-7