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Influence of Co(III) Polypyridyl Complexes on Luminescence Behavior, DNA Binding, Photocleavage, Antimicrobial Activity and Molecular Docking Studies

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Abstract

A new ligand FIPB = 5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid, having three cobalt(III) polypyridyl complexes [Co(phen)2(FIPB)]3+(1) {FIPB = 5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid}, (phen = 1,10-Phenanthroline), [Co(bpy)2(FIPB)]3+(2) (bpy = 2,2’bipyridyl), [Co(dmb)2(FIPB)]3+(3) (dmb = 4, 4′-dimethyl 2, 2′-bipyridine) have been synthesized and characterized by elemental analysis, ES-MS,1H-NMR, 13C-NMR, UV-Vis and FTIR. Their DNA binding behavior has been explored by various spectroscopic titrations and viscosity measurements, which indicated that all the complexes bind to calf thymus DNA by means of intercalation with different binding strengths. The binding properties of these all three complexes towards calf-thymus DNA (CT-DNA) have been investigated by UV-visible, emission spectroscopy and viscosity measurements.The experimental results suggested that three Co(III) complexes can intercalate into DNA base pairs,but with different binding affinities. Photo induced DNA cleavage studies have been performed and results indicate that three complexes efficiently cleave the pBR322-DNA in different forms. The three synthesized compounds were tested for antimicrobial activity by using Staphylococcus aureus and Bacillus subtilis organisms, these results indicated that complex 1 was more activity compared to other two complexes against both tested microbial strains. The in vitro cytotoxicity of these complexes was evaluatedby MTT assay, and complex 1 shows higher cytotoxicity than complex 2 and 3 on HeLa cells.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.All data generated or analyzed during this study are included in this published article.

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Acknowledgements

The authors are grateful to CFRD, Osmania University forspectral analysis.

Funding

The authors are grateful to the UGC-UPE(FAR) ProgramOsmania University, Hyderabad, for funding.

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Authors and Affiliations

  1. Department of Chemistry, UCS, Osmania University, Hyderabad, Telangana State, 500007, India

    Shyam Perka, Ravi Kumar Vuradi, Venugopal Reddy K & Satyanarayana Sirasani

  2. Department of Chemistry, Government Degree College for Women, Karimnagar, Telangana State, 505001, India

    Srinivas Gopu

  3. Department of Chemistry, UCS, Saifabad, Osmania University, Hyderabad, Telangana State, 500007, India

    Navaneetha Nambigari

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  1. Shyam Perka
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Contributions

The author SPconceived this research and designed experiments, wrote the paper and participated in the revisions of it; RKV participated in the design and interpretation of the data helped in writing the paper and participated in the revision of it; SG and VR are participated in analysis of data and revision of paper; NN investigated the molecular docking and interpretation of data; SSN Supervision the research, designed experiments correction of manuscript. The author(s) read and approved the final manuscript.

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Correspondence to Satyanarayana Sirasani.

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Perka, S., Vuradi, R.K., Gopu, S. et al. Influence of Co(III) Polypyridyl Complexes on Luminescence Behavior, DNA Binding, Photocleavage, Antimicrobial Activity and Molecular Docking Studies. J Fluoresc 31, 1009–1021 (2021). https://doi.org/10.1007/s10895-021-02727-y

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