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Inhibition of Quorum Sensing, Motility and Biofilm Formation of Pseudomonas aeruginosa by Copper Oxide Nanostructures

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Abstract

Quorum sensing (QS) is the communication between bacterial cells governed by their population density and regulated by the genes controlling virulence factors and biofilm formation. Multiple mechanisms of biofilms are resistive to antimicrobial chemotherapy; therefore novel strategies are required to overcome its limitations. Here, we report the effect of various copper oxide nanostructures (CuO-NSs) on quorum sensing inhibition. The two-dimensional CuO-NSs such as interlaced nanodiscs, nanodiscs and leaf-shaped nanosheets are prepared via a simple chemical method. The Quorum sensing inhibition (QSI) activity of all the CuO-NS are examined using reporter strain Chromobacterium violaceum CV026 and Escherichia coli pSB1142. We found that the CuO-interlaced nanodisc structures exhibit better QSI activity than nanodiscs and leaf-shaped sheets. The interlaced nanodisc structures are inhibited various long-chain N-acyl homoserine lactones (AHLs) mediated QS individually and confirmed by other QS-associated phenomena for Pseudomonas aeruginosa, including biofilm inhibition, inhibition of virulence factors such as pyocyanin, protease production and swarming motility. Thus QSI activity of CuO-NSs is solely dependent on specific shape offering large surface area and more active sites. The CuO-NS is effective quorum sensing inhibitors, which has potential clinical applications in the management of P. aeruginosa associated infections.

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Acknowledgements

D. D. and P. W. would like to acknowledge the Director, National Centre for Nanosciences and Nanotechnology, and Director, CSIR-National Chemical Laboratory for their valuable support. Mr. Shobhnath Gupta is highly acknowledged for discussion and help in nanomaterials preparation and characterizations.

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Authors and Affiliations

  1. National Centre for Nanoscience and Nanotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai, 400098, India

    Dnyanada G. Desai & Pravin S. Walke

  2. NCIM Resource Centre, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra, 411008, India

    H. Swarali, Govinda R. Navale, A. Prabhune & Mahesh S. Dharne

  3. Centre for Nanoscience & Nanotechnology, Amity University, Mumbai, 410206, India

    Dattatray J. Late

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  1. Dnyanada G. Desai
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  2. H. Swarali
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Correspondence to Mahesh S. Dharne or Pravin S. Walke.

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Desai, D.G., Swarali, H., Navale, G.R. et al. Inhibition of Quorum Sensing, Motility and Biofilm Formation of Pseudomonas aeruginosa by Copper Oxide Nanostructures. J Clust Sci 32, 1531–1541 (2021). https://doi.org/10.1007/s10876-020-01916-2

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  • DOI: https://doi.org/10.1007/s10876-020-01916-2

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