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Stretchable collagen-coated polyurethane-urea hydrogel seeded with bladder smooth muscle cells for urethral defect repair in a rabbit model

  • Special Issue: CESB 2019
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

The major challenge to treat the clinical adverse effects of long-segment urethra is in achieving viable tissue substitution. The substituted construct’s properties-such as its resilience, contraction, and ability to minimize scar-stenosis formation should be considered. In the present work, a unique polyurethane-urea (PUU) fibrous membrane is fabricated by electrospinning. Then PUU was coated by collagen and formed the elasticity hydrogel after immersed in collagen solution. Meanwhile, the cPUU hydrogel exhibited a fibrous microstructure. This cPUU hydrogel had outstanding stretching property with 404 ± 40% elongation at break compared with traditional hydrogels, which satisfied the requirement of urethra. The cPUU hydrogel also supported the adhesion and growth of bladder smooth-muscle cells (BSMCs) in natural state cell morphology. Urethral defects in New Zealand male rabbits were repaired with cPUU seeded with BSMCs in vivo. After three months, more smooth-surface area of reconstructed urethral tissues was observed in the cPUU hydrogel-BMSCs groups compared with that of the control group. The luminal patency and the incidence of complications-including calculus formation, urinary fistula, and urethral-stricture occurrence were significantly lower in the cPUU group compared with that of the control group. Hence, cPUU fibrous hydrogels are promising scaffolds for application in urological tissue engineering.

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Acknowledgements

This study was supported by the National Key R&D Program of China (2016YFC1100203), National Natural Science Foundation of China (81672213, 31872748), Jiangsu Provincial Clinical Orthopedic Center, and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions.

Authors’ contributions

Weiguo Chen, Fengxuan Han and Bin Li conceived and designed the experiments; Professor Mingyu Guo provided the PUU materials; Chunyang Chen and Chengyuan Wang performed the experiments with the help of Fengxuan Han; Chunyang Chen and Chengyuan Wang analyzed the data; Chengyuan Wang and Fengxuan Han wrote the paper.

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Author notes

  1. These authors equally contributed: Chengyuan Wang, Chunyang Chen

Authors and Affiliations

  1. Department of Urology, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215006, China

    Chengyuan Wang, Chunyang Chen & Weiguo Chen

  2. Orthopaedic Institute, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, Jiangsu, 215006, China

    Mingyu Guo, Bin Li & Fengxuan Han

Authors
  1. Chengyuan Wang
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  2. Chunyang Chen
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  3. Mingyu Guo
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  4. Bin Li
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Correspondence to Bin Li, Fengxuan Han or Weiguo Chen.

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Wang, C., Chen, C., Guo, M. et al. Stretchable collagen-coated polyurethane-urea hydrogel seeded with bladder smooth muscle cells for urethral defect repair in a rabbit model. J Mater Sci: Mater Med 30, 135 (2019). https://doi.org/10.1007/s10856-019-6342-7

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