Abstract
Micro-domains of modified surface potential (SP) were created on hydroxyapatite films by direct patterning by mid-energy focused electron beam, typically available as a microprobe of Scanning Electron Microscopes. The SP distribution of these patterns has been studied on sub-micrometer scale by the Kelvin Probe Force Microscopy method as well as lysozyme adsorption. Since the lysozyme is positively charged at physiological pH, it allows us to track positively and negatively charged areas of the SP patterns. Distribution of the adsorbed proteins over the domains was in good agreement with the observed SP patterns.
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Acknowledgments
This project has been funded with support from the European Commission (EC NMP4-SL-2008-212533—BioElectricSurface). This publication reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein. This work was also supported by the Slovak Research and Development Agency under the contract Nos. DO7RP-007-09 and APVV-0199-10, by the Ministry of Education of the Slovak Republic under Contract Nos VEGA 1/0162/10 and VEGA 1/0605/12 and is also the result of the project implementation: 2622020004 supported by the Research & Development Operational Programme funded by the ERDF.
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Plecenik, T., Robin, S., Gregor, M. et al. Directly created electrostatic micro-domains on hydroxyapatite: probing with a Kelvin Force probe and a protein. J Mater Sci: Mater Med 23, 47–50 (2012). https://doi.org/10.1007/s10856-011-4498-x
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DOI: https://doi.org/10.1007/s10856-011-4498-x